| Author | Marques, B. L. C. | |
| Author | Brandão, C. U. | |
| Author | Silva, E. F. | |
| Author | Marques, V. A. | |
| Author | Nogueira, C. A. Villela | |
| Author | Ó, K. M. R. do | |
| Author | Paula, M. T. de | |
| Author | Lewis-Ximenez, L. L. | |
| Author | Lampe, E. | |
| Author | Villar, L. M. | |
| Access date | 2017-01-17T15:19:56Z | |
| Available date | 2017-01-17T15:19:56Z | |
| Document date | 2012 | |
| Citation | MARQUES, B. L. C. et al. Dried Blood Spot Samples: Optimization of Commercial EIAs for Hepatitis C Antibody Detection and Stability Under Different Storage Conditions. Journal of Medical Virology, v.84, p.1600-1607, 2012. | pt_BR |
| ISSN | 0146-6615 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/16869 | |
| Language | eng | pt_BR |
| Publisher | Wiley | pt_BR |
| Rights | restricted access | |
| Subject in Portuguese | Vírus da hepatite C | pt_BR |
| Subject in Portuguese | Diagnóstico | pt_BR |
| Subject in Portuguese | Técnicas Imunoenzimáticas | pt_BR |
| Subject in Portuguese | Estudos de estabilidade | pt_BR |
| Subject in Portuguese | Mancha de sangue seco | pt_BR |
| Title | Dried blood spot samples: optimization of commercial EIAs for hepatitis C antibody detection and stability under different storage conditions | pt_BR |
| Type | Article | |
| DOI | 10.1002/jmv.23379 | |
| Abstract | This study was undertaken to optimize and compare the efficiency of two commercial EIAs for anti-HCV detection (HCV Ab Radim, Pomezzia, Italy and ETI-AB-HCVK-4 DiaSorin, Vercelli, Italy), in dried blood spot (DBS) samples. The long-term stability of anti-HCV on DBS samples stored at three environmental conditions was also evaluated at: 2-8 °C, 20-25 °C, and -20 °C. Paired DBS and serum samples were obtained from individuals with or without anti-HCV. The type of elution buffer, sample and conjugate volume, sample incubation time and cut-off values were evaluated. For both EIAs, a larger sample volume was used, and the cut-off value determined by the manufacturer was employed for Radim EIA; however, ROC curve analysis was used for the DiaSorin EIA. The sensitivity and specificity of Radim EIA on DBS were 97.5% and 99.5%, respectively, and of DiaSorin EIA were 88.9% and 98.9%, respectively. Accurate results were obtained for a period of 117 days using DBS samples stored at all storage conditions, but storage at -20 °C resulted in the lowest variation among the absorbance values. Both EIAs demonstrated the same limit of detection (until dilution of 1:10(4) with estimated viral load of 3.1 × 10(-1) UI/ml), but the Radim EIA was associated with the best performance because a low coefficient of variation was observed in the repetition and reproducibility studies. In conclusion, commercial EIAs can be optimized for anti-HCV detection in DBS samples that are extremely stable at different conditions for more than 100 days. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hepatites Virais. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Affilliation | Hospital Federal dos Servidores do Estado do Rio de Janeiro. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hepatites Virais. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hepatites Virais. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Hospital Universitário Clementino Fraga Filho. Divisão de Hepatologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Hospital Unimed. Petrópolis, RJ, Brasil. | pt_BR |
| Affilliation | Hospital Federal dos Servidores do Estado do Rio de Janeiro. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hepatites Virais. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hepatites Virais. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hepatites Virais. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Subject | hepatitis C virus | pt_BR |
| Subject | diagnosis | pt_BR |
| Subject | dried blood spot | pt_BR |
| Subject | enzyme immunoassay | pt_BR |
| Subject | stability studies | pt_BR |
| e-ISSN | 1096-9071 | |
| Embargo date | 2030-01-01 | |
