Author | Mello, Francisco C. A. | |
Author | Lago, Bárbara V. | |
Author | Lewis-Ximenez, Lia L. | |
Author | Fernandes, Carlos A. | |
Author | Gomes, Selma A. | |
Access date | 2017-01-17T16:03:07Z | |
Available date | 2017-01-17T16:03:07Z | |
Document date | 2012 | |
Citation | MELLO, Francisco C. A. et al. Detection of mixed populations of wild-type and YMDD hepatitis B variants by pyrosequencing in acutely and chronically infected patients. BMC Microbiology, v.12, n.96, 9p, 2012. | pt_BR |
ISSN | 1471-2180 | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/16871 | |
Language | eng | pt_BR |
Publisher | BioMed Central | pt_BR |
Rights | open access | pt_BR |
Subject in Portuguese | Lamivudina | pt_BR |
Subject in Portuguese | Tratamento | pt_BR |
Subject in Portuguese | Infecção crônica pelo vírus da hepatite B (HBV) | pt_BR |
Subject in Portuguese | Terapias antivirais | pt_BR |
Title | Detection of mixed populations of wild-type and YMDD hepatitis B variants by pyrosequencing in acutely and chronically infected patients | pt_BR |
Type | Article | pt_BR |
DOI | 10.1186/1471-2180-12-96 | |
Abstract | Lamivudine (LAM) is associated with the highest known rate of resistance mutations among nucleotide analogs used to treat chronic hepatitis B virus (HBV) infection. Despite this, LAM continues in widespread use, especially in combination therapies. The primary LAM resistance mutation (rtM204V/I) occurs in the YMDD motif of HBV polymerase. The aim of this study was to characterize Brazilian HBV isolates from acute and chronic cases by direct sequencing, and to identify HBV quasispecies in the YMDD motif using a pyrosequencing method capable of detecting single-nucleotide polymorphisms. HBV DNA from serum samples of 20 individuals with acute HBV infection and 44 with chronic infection undergoing antiviral therapies containing LAM were analyzed by direct sequencing and pyrosequencing methods. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Molecular. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Molecular. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Referência Nacional para Hepatites Virais. Rio de Janeiro, RJ. Brasil. | pt_BR |
Affilliation | Laboratório Central de Saúde Pública Noel Nutels, Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Molecular. Rio de Janeiro, RJ, Brasil. | pt_BR |
Subject | hepatitis B | pt_BR |
Subject | treatment | pt_BR |
Subject | chronic hepatitis B virus (HBV) infection | pt_BR |
Subject | YMDD variants | pt_BR |
Subject | antiviral therapies | pt_BR |
Subject | Lamiduvine | pt_BR |