| Author | Cruz, H. M. | |
| Author | Marques, V. A. | |
| Author | Nogueira, C. A. Villela | |
| Author | Ó, K. M. R. do | |
| Author | Lewis-Ximenez, L. L. | |
| Author | Lampe, E. | |
| Author | Villar, L. M. | |
| Access date | 2017-01-17T16:46:43Z | |
| Available date | 2017-01-17T16:46:43Z | |
| Document date | 2012 | |
| Citation | CRUZ, H. M. et al. An evaluation of different saliva collection methods for detection of antibodies against hepatitis C virus (anti-HCV). J Oral Pathol Med., v.41, p.793–800, 2012. | pt_BR |
| ISSN | 0904-2512 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/16873 | |
| Language | eng | pt_BR |
| Publisher | Wiley | pt_BR |
| Rights | restricted access | |
| Subject in Portuguese | Saliva | pt_BR |
| Subject in Portuguese | Vírus da Hepatite C | pt_BR |
| Subject in Portuguese | Diagnóstico | pt_BR |
| Subject in Portuguese | Imunoensaio enzimático | pt_BR |
| Title | An evaluation of different saliva collection methods for detection of antibodies against hepatitis C virus (anti-HCV) | pt_BR |
| Type | Article | |
| DOI | 10.1084/jem.20100388 | |
| Abstract | Vigorous proliferative CD4(+) T cell responses are the hallmark of spontaneous clearance of acute hepatitis C virus (HCV) infection, whereas comparable responses are absent in chronically evolving infection. Here, we comprehensively characterized the breadth, specificity, and quality of the HCV-specific CD4(+) T cell response in 31 patients with acute HCV infection and varying clinical outcomes. We analyzed in vitro T cell expansion in the presence of interleukin-2, and ex vivo staining with HCV peptide-loaded MHC class II tetramers. Surprisingly, broadly directed HCV-specific CD4(+) T cell responses were universally detectable at early stages of infection, regardless of the clinical outcome. However, persistent viremia was associated with early proliferative defects of the HCV-specific CD4(+) T cells, followed by rapid deletion of the HCV-specific response. Only early initiation of antiviral therapy was able to preserve CD4(+) T cell responses in acute, chronically evolving infection. Our results challenge the paradigm that HCV persistence is the result of a failure to prime HCV-specific CD4(+) T cells. Instead, broadly directed HCV-specific CD4(+) T cell responses are usually generated, but rapid exhaustion and deletion of these cells occurs in the majority of patients. The data further suggest a short window of opportunity to prevent the loss of CD4(+) T cell responses through antiviral therapy. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hepatites Virais. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hepatites Virais. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Hospital Universitário Clementino Fraga Filho. Divisão de Hepatologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Hospital Universitário Clementino Fraga Filho. Divisão de Hepatologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hepatites Virais. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hepatites Virais. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hepatites Virais. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Subject | diagnosis | pt_BR |
| Subject | enzyme immunoassay | pt_BR |
| Subject | hepatitis C virus | pt_BR |
| Subject | saliva | pt_BR |
| e-ISSN | 1600-0714 | |
| Embargo date | 2030-01-01 | |
