| Author | Tourinho, Renata Santos | |
| Author | Amado, Luciane Almeida | |
| Author | Villar, Livia Melo | |
| Author | Sampaio, Daniela Vieira | |
| Author | Moraes, Alyne Costa | |
| Author | Ó, Kicia Maria Rodrigues do | |
| Author | Gaspar, Ana Maria Coimbra | |
| Author | Paula, Vanessa Salete de | |
| Access date | 2017-01-17T19:02:03Z | |
| Available date | 2017-01-17T19:02:03Z | |
| Document date | 2011 | |
| Citation | TOURINHO, Renata Santos; et al. Importance of the cutoff ratio for detecting antibodies against hepatitis A virus in a oral fluids by enzyme immunoassay, JOurnal of Virological Methods, v.173, p.169-174, 2011. | pt_BR |
| ISSN | 0166-0934 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/16876 | |
| Language | eng | pt_BR |
| Publisher | Elsevier | pt_BR |
| Rights | restricted access | |
| Subject in Portuguese | Fluido oral | pt_BR |
| Subject in Portuguese | Anticorpos anti-HAV totais | pt_BR |
| Subject in Portuguese | Hepatite A | pt_BR |
| Subject in Portuguese | Ensaios baseados em soro | pt_BR |
| Title | Importance of the cutoff ratio for detecting antibodies against hepatitis A virus in oral fluids by enzyme immunoassay | pt_BR |
| Type | Article | |
| DOI | 10.1016/j.jviromet.2011.01.014 | |
| Abstract | Multiple studies have examined the use of oral fluids in modified serum-based assays aiming to replace serum in antibody detection for hepatitis A. However, the reliable detection of HAV immunity in oral fluid requires an extremely sensitive assay; most immunoassays designed for serum antibody determination lack sufficient sensitivity for this purpose. Consequently, an "in-house" competitive enzyme immunoassay (EIA) designed specifically for use with oral samples collected using a ChemBio(®) device was developed to detect total anti-HAV antibodies (IgG and IgM). This system was compared to an in-house competitive EIA and a commercial EIA considered to be the "gold standard" using corresponding serum samples (n=225) to determine the accuracy of the assay and to evaluate the importance of the cutoff ratio for the detection of anti-HAV antibodies in oral fluids. When the median serum cutoff and the optimal oral fluid cutoff (ROC analysis) obtained from the in-house competitive EIA were compared, the oral fluid cutoff was found to be 28.8% higher than the serum cutoff. When different oral fluid cutoff values were compared, a reduction of about 17% was shown to be essential to increase test accuracy. At an oral fluid cutoff value of 0.351, sensitivity and specificity were higher, reaching 91.7% and 86.2% (p<0.001, AUROC=0.915), respectively. The convenience, accuracy and non-invasive nature of the developed method make it a useful alternative to serum-based assays for discriminating between HAV-immune and non-immune individuals. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Desenvolvimento Tecnológico em Virologia. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Desenvolvimento Tecnológico em Virologia. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hepatites Virais. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Desenvolvimento Tecnológico em Virologia. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Desenvolvimento Tecnológico em Virologia. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Affilliation | Hospital UNIMED. Petrópolis, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Desenvolvimento Tecnológico em Virologia. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Desenvolvimento Tecnológico em Virologia. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Subject | Oral fluid | pt_BR |
| Subject | Total anti-HAV antibodies | pt_BR |
| Subject | In-house competitive EIA | pt_BR |
| Subject | hepatitis A. | pt_BR |
| Subject | serum-based assays | pt_BR |
| e-ISSN | 1879-0984 | |
| Embargo date | 2030-01-01 | |
| xmlui.metadata.dc.subject.ods | 11 Cidades e comunidades sustentáveis | |
