| Author | Majerowicz, Selma | |
| Author | Grief, Christopher | |
| Author | Pinto, Marcelo Alves | |
| Author | Baptista, Marcia Leite | |
| Author | Pinhão, Angela Teixeira | |
| Author | Airano, Renata Cristina | |
| Author | Vitral, Cláudia Lamarca | |
| Author | Marchevsky, Renato Sergio | |
| Author | Yoshida, Clara F. T. | |
| Author | Barth, Ortrud Monika | |
| Access date | 2017-01-18T11:35:29Z | |
| Available date | 2017-01-18T11:35:29Z | |
| Document date | 2010 | |
| Citation | MAJEROWICZ, Selma; et al. Ultrastructural localization of Hepatitis C Virus positive and negative strand RNA and proteins in Hepatocytes of a Rhesus Monkey (Macaca Mulatta). Virus Reviews & Research, v.14, n.1, 16p, 2009. | pt_BR |
| ISSN | 1519-2563 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/16884 | |
| Language | eng | pt_BR |
| Publisher | Brazilian Society of Virology | pt_BR |
| Rights | open access | |
| Subject in Portuguese | Vírus da hepatite C | pt_BR |
| Subject in Portuguese | Macaco rhesus | pt_BR |
| Subject in Portuguese | Ultraestrutura | pt_BR |
| Subject in Portuguese | Hibridização in situ | pt_BR |
| Subject in Portuguese | Proteínas virais | pt_BR |
| Title | Ultrastructural localization of Hepatitis C virus positive and negative strand RNA and proteins in Hepatocytes of a Rhesus Monkey (Macaca Mulatta) | pt_BR |
| Type | Article | |
| Abstract | Morphological alterations of the liver from rhesus macaque (Macaca mulatta)
experimentally infected with hepatitis C virus (HCV) were analyzed using electron
microscopy. The localization of viral RNA and proteins inside hepatocytes was
demonstrated using in situ hybridization and immunoelectron microscopy techniques.
The animals were inoculated by different routes. The infection was successful only by
use of the intrasplenic approach to HCV infected autogenic hepatocyte transplant. The
inoculum used to infect the hepatocytes was characterized as genotype 3 with 107 RNA
copies/mL. In situ hybridization was performed using a complementary negative and
positive strand probe made with the specific primer. Despite that the level of HCV
infection was considered to be low, we were able to detect and localize viral positive
and negative RNA strands and viral proteins in altered membranes of the rough
endoplasmic reticulum in infected liver cells, showing evidence of viral replication in
vivo. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ. Brasil / NIBSC. Potters Bar. Herts, EN6 3QG, Inglaterra. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Affilliation | Universidade Federal Fluminense. Instituto Biomédico. Niterói, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto de Tecnologia em Imunobiológicos. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Subject | rhesus monkey | pt_BR |
| Subject | hepatitis C virus | pt_BR |
| Subject | ultrastructure | pt_BR |
| Subject | in situ hybridization | pt_BR |
| Subject | viral proteins | pt_BR |
| Subject | replication complex | pt_BR |
| e-ISSN | 2357-9323 | |
