| Author | Scalioni, Letícia de Paula | |
| Author | Cruz, Helena Medina | |
| Author | Paula, Vanessa Salete de | |
| Author | Oliveira, Jaqueline Corrêia | |
| Author | Santos, Renata Tourinho dos | |
| Author | Castro, Ana Rita Coimbra Motta | |
| Author | Murat, Paula Guerra | |
| Author | Nogueira, Cristiane Alves Villella | |
| Author | Lewis-Ximenez, Lia Laura | |
| Author | Lampe, Elisabeth | |
| Author | Villar, Livia Melo | |
| Access date | 2017-01-24T10:52:07Z | |
| Available date | 2017-01-24T10:52:07Z | |
| Document date | 2013 | |
| Citation | SCALIONI, Letícia de Paula; et al. Importance of Collection Methods and Stability of Oral Fluid Samples for Hepatitis B Surface Antigen Detection. Journal of Clinical Laboratory Analysis, v. 27, p.186–194, 2013. | pt_BR |
| ISSN | 0887-8013 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/16912 | |
| Language | eng | pt_BR |
| Publisher | Wiley | pt_BR |
| Rights | restricted access | |
| Subject in Portuguese | Vírus da hepatite B | pt_BR |
| Subject in Portuguese | Fluido oral | pt_BR |
| Subject in Portuguese | Diagnóstico | pt_BR |
| Subject in Portuguese | Estudo de estabilidade | pt_BR |
| Subject in Portuguese | Dispositivo de coleta | pt_BR |
| Title | Importance of collection methods and stability of oral fluid samples for hepatitis B surface antigen detection | pt_BR |
| Type | Article | |
| DOI | 10.1002/jcla.21582 | |
| Abstract | Oral fluid (OF) sample collection
and stability for HBsAg detection are not
fully established. This study aims to investigate
the applicability of OF collectors and
sample stability for Hepatitis B virus surface
antigen detection. Methods: Paired serum
and OF samples were obtained from 191
individuals, and Chembio (Chembio Diagnostic
System, USA) and Salivette (Sarstedt,
Germany) devices were used for OF
collection. Two HBsAg enzyme immunoassays
(EIAs) were used (HBsAg One kit,
Radim, Rome, Italy and ETI-MAK-4, Dia-
Sorin, Vercelli, Italy) to determine the most
efficient method according OF collector.
Sample volume, incubation time, and cutoff
(CO) value were evaluated. The stability of
OF samples was determined under different
environmental conditions. Results: Chembio
samples analyzed using DiaSorin EIA
without modification of the manufacturer’s
instructions, demonstrated a sensitivity of
95.24% and a specificity of 100%. Salivette
samples analyzed with Radim EIA with receiver
operating characteristic (ROC) curve
for calculating the CO showed a sensitivity
of 78.26% and a specificity of 89.88%. HBsAg
was detected in Chembio and Salivette
samples under different environmental conditions,
but the Chembio samples were the
most stable. Conclusions: Both collectors
can be used for HBsAg detection in OF samples,
but some modifications of commercial
EIAs should be incorporated for Salivette
device. OF samples were reliably stable
and could be stored for up to 90 days at
2–8◦C.. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hepatites Virais. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hepatites Virais. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Desenvolvimento Tecnológico em Virologia. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hepatites Virais. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Desenvolvimento Tecnológico em Virologia. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Affilliation | Universidade Federal de Mato Grosso do Sul. Laboratório de Imunologia Clínica. Campo Grande, MS, Brasil. | pt_BR |
| Affilliation | Universidade Federal de Mato Grosso do Sul. Laboratório de Imunologia Clínica. Campo Grande, MS, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Faculdade de Medicina. Hospital Universitário Clementino Fraga Filho. Divisão de Hepatologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hepatites Virais. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hepatites Virais. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hepatites Virais. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Subject | hepatitis B virus | pt_BR |
| Subject | Oral fluid | pt_BR |
| Subject | diagnosis | pt_BR |
| Subject | stability study | pt_BR |
| Subject | collection device | pt_BR |
| e-ISSN | 1098-2825 | |
| Embargo date | 2030-01-01 | |
