| Author | Azevedo, Maria Isabel N. Di | |
| Author | Knoff, Marcelo | |
| Author | Carvalho, Vitor L. | |
| Author | Mello, Wildon N. | |
| Author | Torres, Eduardo J. Lopes | |
| Author | Gomes, Delir C. | |
| Author | Iñiguez, Alena M. | |
| Access date | 2017-01-24T12:26:10Z | |
| Available date | 2017-01-24T12:26:10Z | |
| Document date | 2015 | |
| Citation | AZEVEDO, Maria Isabel N. Di; et al. Morphological and genetic identification of Anisakis paggiae (Nematoda: Anisakidae) in dwarf sperm whale Kogia sima from Brazilian waters. Diseases of Aquatic Organims, v.113, p.103-111, Mar. 2015. | pt_BR |
| ISSN | 0177-5103 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/16913 | |
| Language | eng | pt_BR |
| Publisher | Inter Research | pt_BR |
| Rights | open access | |
| Subject in Portuguese | Cetáceo | pt_BR |
| Subject in Portuguese | Nematóide parasita | pt_BR |
| Subject in Portuguese | Microscopia de luz | pt_BR |
| Subject in Portuguese | Microscopia eletrônica de varredura | pt_BR |
| Subject in Portuguese | mtDNA cox2 | pt_BR |
| Subject in Portuguese | Brasil | pt_BR |
| Title | Morphological and genetic identification of Anisakis paggiae (Nematoda: Anisakidae) in dwarf sperm whale Kogia sima from Brazilian waters | pt_BR |
| Type | Article | |
| DOI | 10.3354/dao02831 | |
| Abstract | Anisakid nematodes have been identified in a wide variety of fish and marine mammal species. In Brazil, Anisakis physeteris, A. insignis, A. typica, A. nascetti, and those of the A. simplex complex have been reported infecting fishes and cetaceans. In this study, specimens collected from a dwarf sperm whale Kogia sima (Owen, 1866) stranded on the northeastern coast of Brazil were identified through morphological and genetic analyses as A. paggiae. Anisakids were examined through differential interference contrast light and scanning electron microscopy (SEM). Morphological and morphometric analysis revealed that these specimens belonged to Anisakis sp. clade II and more specifically to A. paggiae, exhibiting a violin-shaped ventriculus and 3 denticulate caudal plates, which are taxonomic characters considered unique to this species. Genetic analysis based on the mtDNA cox2 gene confirmed our identification of A. paggiae. Phylogenetic trees using both maximum likelihood and neighbor-joining methods revealed a strongly supported monophyletic clade (bootstrap support = 100%) with all available A. paggiae sequences. Integrative taxonomic analysis allowed the identification of A. paggiae for the first time in Brazilian waters, providing new data about their geographical distribution. Moreover, here we present the first SEM images of this species. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia de Tripanossomatídeos. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Helmintos Parasitos de Vertebrados. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Affilliation | Associação de Pesquisa e Preservação de Ecossistemas Aquáticos — AQUASIS. Caucaia, CE, Brasil. | pt_BR |
| Affilliation | UERJ. Faculdade de Ciências Médicas. Laboratório de Helmintologia Roberto Lascasas Porto. Rio de Janeiro, RJ, Brasil / UFRJ. Instituto Nacional de Ciência e Tecnologia em Biologia Estrutural e Bioimagens. Rio de Janeiro, RJ, Brasil / UFRJ. Laboratório de Ultraestrutura Celular Hertha Meyer, Instituto de Biofísica Carlos Chagas Filho. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Helmintos Parasitos de Vertebrados. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia de Tripanossomatídeos. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Subject | Cetacean | pt_BR |
| Subject | Parasitic nematode | pt_BR |
| Subject | Scanning electron microscopy | pt_BR |
| Subject | Light microscopy | pt_BR |
| Subject | mtDNA cox2 | pt_BR |
| Subject | Brazil | pt_BR |
| e-ISSN | 1616-1580 | |
