Author | Cunha, Joana Paixão Monteiro | |
Author | Araujo, Adriano Fernando da Silva | |
Author | Santos, Edson | |
Author | Castro Filho, Bernardo Galvão | |
Author | Alcantara, Luiz Carlos Júnior | |
Access date | 2011-03-26T23:00:19Z | |
Available date | 2011-03-26T23:00:19Z | |
Document date | 2011 | |
Citation | MONTEIRO-CUNHA, J. P. et al. Lack of high-level resistance mutations in HIV Type 1 BF recombinant strains circulating in northeast Brazil. AIDS Research and Human Retroviruses, v. 27, n.6, p. 623-631, 2011 | pt_BR |
ISSN | 0889-2229 | pt_BR |
xmlui.metadata.dc.identifier.other | 21087197 | |
URI | https://www.arca.fiocruz.br/handle/icict/1760 | |
Language | eng | pt_BR |
Rights | open access | en |
Subject in Portuguese | DRAM | pt_BR |
Subject in Portuguese | HIV-1 | pt_BR |
Subject in Portuguese | Sequenciamento genetico | pt_BR |
Subject in Portuguese | Resisencia à drogas | pt_BR |
Title | Lack of high-level resistance mutations in HIV Type 1 BF recombinant strains circulating in northeast Brazil | pt_BR |
Type | Article | pt_BR |
Abstract | The genetic variability and the prevalence of drug resistance-associated mutations (DRAM) of HIV-1 isolates from 50 women and 8 children from Feira de Santana, Bahia, Brazil were investigated. DNA samples were obtained and pol sequences were generated by PCR and direct sequencing. Phylogenetic analysis showed that 39 (67.2%) samples were subtype B, four (6.9%) F, one (1.7%) C, and 14 (24.1%) BF recombinants. Four different BF recombination patterns were detected. Twelve (20.7%) samples shared the same breakpoint within the reverse transcriptase (RT) sequence. Fifty-five (94.8%) isolates showed several resistance-associated mutations in the RT and the protease (PR) genes. Ten (17.2%) isolates presented mutations associated with a high level of resistance: nine (15.5%) to nucleoside RT inhibitors (NRTI), four (6.9%) to nonnucleoside RT inhibitors (NNRTI), and three (5.2%) to PR inhibitors (PIs). Subtype B-infected patients had, on average, 0.5 high-level DRAM per sequence while no mutations were observed in BF recombinants, although the two groups were under ARV for a similar period of time. Our data indicate the predominance of the subtype B, followed by BF recombinants in this population, and the dissemination of a recombinant strain in Bahia, which could be related to adaptive advantages of these variants over the predominant subtype B. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
Affilliation | Escola Bahiana de Medicina e Saúde Pública. Salvador, BA, Brasil | pt_BR |
Affilliation | National Cancer Institute. National Institutes of Health. Vaccine Branch. Bethesda, Maryland, USA | pt_BR |
DeCS | Resistência a Medicamentos | pt_BR |
DeCS | HIV-1 | pt_BR |
DeCS | Análise Mutacional de DNA | pt_BR |
DeCS | Reação em Cadeia da Polimerase | pt_BR |