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https://www.arca.fiocruz.br/handle/icict/17629
Tipo de documento
ArtigoDireito Autoral
Acesso restrito
Data de embargo
2030-01-01
Coleções
- IOC - Artigos de Periódicos [12642]
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PHEROMONE GLAND DEVELOPMENT AND PHEROMONE PRODUCTION IN LUTZOMYIA LONGIPALPIS (DIPTERA: PSYCHODIDAE: PHLEBOTOMINAE)
Lutzomyia
pheromone gland development
ultrastructure
pheromone production
Autor(es)
Afiliação
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular de Insetos. Rio de Janeiro, RJ. Brasil / Universidade Federal Fluminense. Departamento de Biologia Celular e Molecular. Niterói, RJ, Brasil.
Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Parasitologia. Belo Horizonte, MG, Brasil.
Universidade Federal Fluminense. Departamento de Biologia Celular e Molecular. Niterói, RJ, Brasil.
Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Parasitologia. Belo Horizonte, MG, Brasil.
Rothamsted Research. Department of Biological Chemistry. Harpenden, United Kingdom.
Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Parasitologia. Belo Horizonte, MG, Brasil.
Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.
Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Parasitologia. Belo Horizonte, MG, Brasil.
Universidade Federal Fluminense. Departamento de Biologia Celular e Molecular. Niterói, RJ, Brasil.
Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Parasitologia. Belo Horizonte, MG, Brasil.
Rothamsted Research. Department of Biological Chemistry. Harpenden, United Kingdom.
Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Parasitologia. Belo Horizonte, MG, Brasil.
Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.
Resumo em Inglês
The sand ßy Lutzomyia longipalpis (Lutz & Neiva) (Diptera: Psychodidae: Phlebotominae)
is the main vector of American visceral leishmaniasis. Adult males produce a terpenoid sex
pheromone that in some cases also acts as male aggregation pheromone. We have analyzed the
correlation between male pheromone production levels and pheromone gland cell morphogenesis
after adult emergence from pupae. The abdominal tergites of L. longipalpis males were dissected and
Þxed in glutaraldehyde for transmission electron microscopy, or the pheromone was extracted in
analytical grade hexane. Pheromone chemical analysis was carried out at 3- to 6-h intervals during the
Þrst 24 h after emergence and continued daily until the seventh day. All extracts were analyzed by
gas chromatography. For the morphological analysis, we used insects collected at 0 Ð 6, 9 Ð12, 12Ð14,
and 96 h after emergence. Ultrastructural data from 0- to 6-h-old adult males revealed smaller
pheromone gland cells with small microvilli at the end apparatus. Lipid droplets and peroxisomes were
absent or very rare, but a large number of mitochondria could be seen. Lipid droplets started to appear
in the gland cells cytoplasm 9 h after adult emergence, and their number and size increased with
age, together with the presence of several peroxisomes, suggesting a role for these organelles in
pheromone biosynthesis. At 12Ð15 h after emergence, the lipid droplets were mainly distributed near
the microvilli but were smaller than those in mature older males (4 d old). Pheromone biosynthesis
started around 12 h after emergence and increased continuously during the Þrst 3 d, stabilizing
thereafter, coinciding with the period when males are more able to attract females.
Palavras-chave em inglês
sand fliesLutzomyia
pheromone gland development
ultrastructure
pheromone production
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