| Author | Gomes, Harrison Magdinier | |
| Author | Elias, Atina Ribeiro | |
| Author | Oelemann, Maranibia Aparecida Cardoso | |
| Author | Pereira, Márcia Aparecida da Silva | |
| Author | Montes, Fátima Fandinho Onofre | |
| Author | Marsico, Ana Grazia | |
| Author | Kritski, Afrânio Lineu | |
| Author | Filho, Luciano dos Anjos | |
| Author | Caldas, Paulo C. | |
| Author | Possuelo, Lia Gonçalves | |
| Author | Cafrune, Patrícia | |
| Author | Rossetti, Maria Lúcia | |
| Author | Lucena, Norma | |
| Author | Saad, Maria Helena Feres | |
| Author | Cavalcanti, Hebe Rodrigues | |
| Author | Leite, Clarisse Queico Fujimura | |
| Author | Brito, Rossana Coimbra de | |
| Author | Lopes, Maria Luiza | |
| Author | Lima, Karla | |
| Author | Souza, Maisa | |
| Author | Trindade, Rita de Cássia | |
| Author | Zozio, Thierry | |
| Author | Sola, Christophe | |
| Author | Rastogi, Nalin | |
| Author | Suffys, Philip Noel | |
| Access date | 2017-01-31T11:59:15Z | |
| Available date | 2017-01-31T11:59:15Z | |
| Document date | 2012 | |
| Citation | GOMES, Harrison Magdinier; et al. Spoligotypes of Mycobacterium tuberculosis complex isolates from patients residents of 11 states of Brazil. Infection, Genetics and Evolution, v.12, n.4, p.649–656, June 2012. | pt_BR |
| ISSN | 1567-1348 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/17631 | |
| Language | eng | pt_BR |
| Publisher | Elsevier | pt_BR |
| Rights | restricted access | |
| Subject in Portuguese | Mycobacterium tuberculosis | pt_BR |
| Subject in Portuguese | Brasil | pt_BR |
| Subject in Portuguese | Estrutura populacional | pt_BR |
| Subject in Portuguese | Base de dados | pt_BR |
| Title | Spoligotypes of Mycobacterium tuberculosis complex isolates from patients residents of 11 states of Brazil | pt_BR |
| Type | Article | |
| DOI | 10.1016/j.meegid.2011.08.027 | |
| Abstract | One of the high tuberculosis (TB) incidence countries in the world, Brazil is characterized by considerable differences in TB incidence on regional and state level. In the present study, we describe Brazilian spoligotypes of 1991 Mycobacterium tuberculosis complex (MTC) clinical isolates from patients residents of 11 states from different regions of the country, diagnosed between 1996 and 2005. By performing spoligotyping on a large number of M. tuberculosis clinical isolates, one of the main objectives of this study was to determine the major genotype families causing TB in Brazil and to verify the region-associated genotype distribution. We observed a total of 577 distinct spoligopatterns, 12.6% of these corresponded to orphan patterns while 87.4% belonged to 326 shared-types (SITs). Among the latter, 86 SITs (isolated from 178 patients) had been observed for the first time in this study, the most frequent being SIT2517 which belonged to the T3-ETH lineage and was exclusively found among patients residents of Belém, the capital of the state of Pará (n=8 isolates). Irrespective of shared-type labeling, a total of 19.5% strains were unique (unclustered) in our study as opposed to 80.5% clustered isolates (189 clusters, size range from 2 to 205 isolates). The three largest clusters were SIT42 of the Latin-America & Mediterranean (LAM) 9 clade (10.3%), SIT53 of the T clade (7.6%), and SIT50 of the Haarlem clade (5.4%). The predominant MTC lineages in Brazil in decreasing order belonged to the LAM (46%); the ill-defined T (18.6%); the Haarlem (12.2%), the X (4.7%), the S (1.9%), and the East African Indian (EAI) (0.85%) families. The rest of clades grouped together as Mycobacterium africanum, Mycobacterium bovis, Beijing, Central Asian (CAS), and the Manu types, represented less than 1% of the strains. Finally, about 15% of the isolates showed spoligotype signatures that were not yet classified among well-defined lineages. In conclusion, we provide hereby a first insight into the population structure of MTC isolates in Brazil, showing the predominance of both LAM and T family and the existence of region-associated genotypes. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular Aplicada em Micobactérias, . Rio de Janeiro, RJ. Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular Aplicada em Micobactérias, . Rio de Janeiro, RJ. Brasil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular Aplicada em Micobactérias, . Rio de Janeiro, RJ. Brasil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular Aplicada em Micobactérias, . Rio de Janeiro, RJ. Brasil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular Aplicada em Micobactérias, . Rio de Janeiro, RJ. Brasil | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Doenças do Tótax. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Doenças do Tótax. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Doenças do Tótax. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Ministério da Saúde. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Rio Grande do Sul. Porto Alegre, RS, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Rio Grande do Sul. Porto Alegre, RS, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Rio Grande do Sul. Porto Alegre, RS, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisa Aggeu Magalhães. PE, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular Aplicada em Micobactérias, . Rio de Janeiro, RJ. Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular Aplicada em Micobactérias, . Rio de Janeiro, RJ. Brasil. | pt_BR |
| Affilliation | Universidade Estadual de São Paulo. Departamento de Farmácia. São Paulo, SP, Brasil. | pt_BR |
| Affilliation | Universidade do Estado do Rio de Janeiro, Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Instituto Evandro Chagas. PA, Brasil. | pt_BR |
| Affilliation | Instituto Evandro Chagas. PA, Brasil. | pt_BR |
| Affilliation | Instituto Evandro Chagas. PA, Brasil. | pt_BR |
| Affilliation | Universidade Federal de Sergipe. SE, Brasil. | pt_BR |
| Affilliation | Institut Pasteur de la Guadeloupe. WHO Supranational TB Reference Laboratory. Abymes, France. | pt_BR |
| Affilliation | Institut Pasteur de la Guadeloupe. WHO Supranational TB Reference Laboratory. Abymes, France. | pt_BR |
| Affilliation | Institut Pasteur de la Guadeloupe. WHO Supranational TB Reference Laboratory. Abymes, France. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular Aplicada em Micobactérias, . Rio de Janeiro, RJ. Brasil. | pt_BR |
| Subject | Brazil | pt_BR |
| Subject | Population structure | pt_BR |
| Subject | Spoligotyping | pt_BR |
| Subject | Database | pt_BR |
| e-ISSN | 1567-7257 | |
| Embargo date | 2030-01-01 | |
