| Author | Regis, Eduardo G. | |
| Author | Souza, Victor Barreto de | |
| Author | Morgado, Mariza G. | |
| Author | Bozza, Marcelo T. | |
| Author | Leng, Lin | |
| Author | Bucala, Richard | |
| Author | Bou-Habib, Dumith C. | |
| Access date | 2017-02-07T10:49:09Z | |
| Available date | 2017-02-07T10:49:09Z | |
| Document date | 2010 | |
| Citation | REGIS, Eduardo G. et al. Elevated levels of macrophage migration inhibitory factor (MIF) in the plasma of HIV-1-infected patients and in HIV-1-infected cell cultures: A relevant role on viral replication. Virology, v.399, n.1, p.31-38, Mar. 2010. | pt_BR |
| ISSN | 0042-6822 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/17687 | |
| Language | eng | pt_BR |
| Publisher | Elsevier | pt_BR |
| Rights | open access | |
| Subject in Portuguese | AIDS | pt_BR |
| Subject in Portuguese | HIV-1 | pt_BR |
| Title | Elevated levels of macrophage migration inhibitory factor (MIF) in the plasma of HIV-1-infected patients and in HIV-1-infected cell cultures: a relevant role on viral replication | pt_BR |
| Type | Article | |
| DOI | 10.1016/j.virol.2009.12.018 | |
| Abstract | The cytokine macrophage migration inhibitory factor (MIF) is involved in the pathogenesis of inflammatory and infectious diseases, however its role in HIV-1 infection is unknown. Here we show that HIV-1-infected patients present elevated plasma levels of MIF, that HIV-1-infected peripheral blood mononuclear cells (PBMCs) release a greater amount of MIF, and that the HIV-1 envelope glycoprotein gp120 induces MIF secretion from uninfected PBMCs. The HIV-1 replication in PBMCs declines when these cells are treated with anti-MIF antibodies, and exposure of HIV-1-infected cells to the ABC-transporter inhibitor probenecid results in inhibition of MIF secretion. The addition of recombinant MIF (rhMIF) to HIV-1-infected PBMCs enhances viral replication of CCR5- or CXCR4-tropic HIV-1 isolates. Using a T CD4(+) cell lineage containing an HIV long terminal repeats (LTR)-Luciferase construct, we detected that rhMIF promotes transcription from HIV-1 LTR. Our results show that HIV-1 induces MIF secretion and suggest that MIF influences the HIV-1 biology through activation of HIV-1 LTR. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa sobre o Timo. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa sobre o Timo. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de AIDS e Imunologia Molecular. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Affilliation | Universidade Federa do Rio de Janeiro. Instituto de Microbiologia. Departamento de Imunologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Yale School of Medicine. CT, USA. | pt_BR |
| Affilliation | Yale School of Medicine. CT, USA. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa sobre o Timo. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Subject | MIF | pt_BR |
| Subject | HIV-1 | pt_BR |
| Subject | AIDS | pt_BR |
| Subject | LTR | pt_BR |
| Subject | gp120 | pt_BR |
