Mostrar el registro sencillo del ítem

AutorLakshmikanth, Chikkamenahalli Lakshminarayana
AutorPetsel Jacob, Shancy
AutorKudva, Avinash Kundadka
AutorLatchoumycandane, Calivarathan
AutorYashaswini, Puttaraju Srikanta Murthy
AutorSumanth, Mosale Seetharam
AutorAlbuquerque, Cassiano F. Gonçalves de
AutorSilva, Adriana R.
AutorSingh, Sridevi Annapurna
AutorFaria Neto, Hugo C. Castro
AutorPrabhu, Sandeep Kumble
AutorMcIntyre, Thomas M.
AutorMarathe, Gopal Kedihithlu
Fecha de acceso2017-03-01T14:45:23Z
Fecha de disponibilización2017-03-01T14:45:23Z
Fecha de publicación2016
ReferenciaLakshmikanth, Chikkamenahalli Lakshminarayan; et al. Escherichia coli Braun Lipoprotein (BLP) exhibits endotoxemia – like pathology in Swiss albino mice. Sci Rep., v.6:34666, 13p, Oct. 2016.pt_BR
ISSN2045-2322pt_BR
URIhttps://www.arca.fiocruz.br/handle/icict/17908
Idiomaengpt_BR
EditorNature Publishing Grouppt_BR
Derechos de autoropen access
Palabras clave en PortuguésEscherichia colipt_BR
Palabras clave en Portuguésendotoxemiapt_BR
Palabras clave en PortuguésLipoproteínaspt_BR
Palabras clave en PortuguésCamundongospt_BR
TítuloEscherichia coli Braun Lipoprotein (BLP) exhibits endotoxemia - like pathology in Swiss albino micept_BR
Tipo del documentoArticle
DOI10.1038/srep34666
Resumen en InglésThe endotoxin lipopolysaccharide (LPS) promotes sepsis, but bacterial peptides also promote inflammation leading to sepsis. We found, intraperitoneal administration of live or heat inactivated E. coli JE5505 lacking the abundant outer membrane protein, Braun lipoprotein (BLP), was less toxic than E. coli DH5α possessing BLP in Swiss albino mice. Injection of BLP free of LPS purified from E. coli DH5α induced massive infiltration of leukocytes in lungs and liver. BLP activated human polymorphonuclear cells (PMNs) ex vivo to adhere to denatured collagen in serum and polymyxin B independent fashion, a property distinct from LPS. Both LPS and BLP stimulated the synthesis of platelet activating factor (PAF), a potent lipid mediator, in human PMNs. In mouse macrophage cell line, RAW264.7, while both BLP and LPS similarly upregulated TNF-α and IL-1β mRNA; BLP was more potent in inducing cyclooxygenase-2 (COX-2) mRNA and protein expression. Peritoneal macrophages from TLR2(-/-) mice significantly reduced the production of TNF-α in response to BLP in contrast to macrophages from wild type mice. We conclude, BLP acting through TLR2, is a potent inducer of inflammation with a response profile both common and distinct from LPS. Hence, BLP mediated pathway may also be considered as an effective target against sepsis.pt_BR
AfiliaciónUniversity of Mysore. Department of Studies in Biochemistry. Karnataka, India.pt_BR
AfiliaciónUniversity of Mysore. Department of Studies in Biochemistry. Karnataka, India.pt_BR
AfiliaciónThe Pennsylvania State University. Center for Molecular Immunology and Infectious Disease and Center for Molecular Toxicology and Carcinogenesis. Department of Veterinary and Biomedical Sciences. University Park, PA, USA.pt_BR
AfiliaciónCleveland Clinic Lerner Research Institute. Department of Cellular and Molecular Medicine. Cleveland, Ohio, USA.pt_BR
AfiliaciónCentral Food Technological Research Institute/CSIR. Department of Protein Chemistry & Technology. Karnataka, India.pt_BR
AfiliaciónUniversity of Mysore. Department of Studies in Biochemistry. Karnataka, India.pt_BR
AfiliaciónFundação Oswado Cruz. Instituto Oswaldo Cruz. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ, Brasil.pt_BR
AfiliaciónFundação Oswado Cruz. Instituto Oswaldo Cruz. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ, Brasil.pt_BR
AfiliaciónUniversity of Mysore. Department of Studies in Biochemistry. Karnataka, India.pt_BR
AfiliaciónFundação Oswado Cruz. Instituto Oswaldo Cruz. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ, Brasil.pt_BR
AfiliaciónThe Pennsylvania State University. Center for Molecular Immunology and Infectious Disease and Center for Molecular Toxicology and Carcinogenesis. Department of Veterinary and Biomedical Sciences. University Park, PA, USA.pt_BR
AfiliaciónCleveland Clinic Lerner Research Institute. Department of Cellular and Molecular Medicine. Cleveland, Ohio, USA.pt_BR
AfiliaciónUniversity of Mysore. Department of Studies in Biochemistry. Karnataka, India.pt_BR
Palavras clave en InglêsEscherichia coli Braun Lipoprotein (BLP)pt_BR
Palavras clave en InglêsSwiss albino micept_BR
Palavras clave en Inglêsendotoxemiapt_BR


Ficheros en el ítem

application/pdf
Nombre:
hugo3_farianeto_etal_IOC_2016.pdf
Tamaño:
1.868Mb
Formato:
application/
Ver/Abrir
application/msword
Nombre:
srep34666-s1.doc
Tamaño:
1.437Mb
Formato:
application/
Descripción:
Material Suplementar
Ver/Abrir

Este ítem aparece en la(s) siguiente(s) colección(ones)

Mostrar el registro sencillo del ítem