| Author | Souza, Bruno Solano de Freitas | |
| Author | Sampaio, Gabriela Louise de Almeida | |
| Author | Pereira, Ciro Silveira e | |
| Author | Campos, Gubio Soares | |
| Author | Sardi, Silvia I | |
| Author | Freitas, Luiz Antonio Rodrigues de | |
| Author | Figueira, Claudio Pereira | |
| Author | Paredes, Bruno D | |
| Author | Nonaka, Carolina K V | |
| Author | Azevedo, Carine Machado | |
| Author | Rocha, Vinicius Pinto Costa | |
| Author | Bandeira, Antonio Carlos de Albuquerque | |
| Author | Otero, Rosalia Mendez | |
| Author | Santos, Ricardo Ribeiro dos | |
| Author | Soares, Milena Botelho Pereira | |
| Access date | 2017-03-03T17:45:57Z | |
| Available date | 2017-03-03T17:45:57Z | |
| Document date | 2016 | |
| Citation | SOUZA, B. S. F. et al. Zika virus infection induces mitosis abnormalities and apoptotic cell death of human neural progenitor cells. Scientific Reports, v. 6, p. 39775, 2016. | pt_BR |
| ISSN | 2045-2322 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/17924 | |
| Sponsorship | CAPES (Zika FastTrack), FINEP, FAPERJ and FAPESB. | pt_BR |
| Language | eng | pt_BR |
| Publisher | Nature Publishing Group | pt_BR |
| Rights | open access | pt_BR |
| Subject in Portuguese | Zika virus | pt_BR |
| Subject in Portuguese | Infecção | pt_BR |
| Subject in Portuguese | Cérebro | pt_BR |
| Subject in Portuguese | Anormalidades do Sistema Nervoso | pt_BR |
| Subject in Portuguese | Humanos | pt_BR |
| Subject in Portuguese | Microcefalia | pt_BR |
| Title | Zika virus infection induces mitosis abnormalities and apoptotic cell death of human neural progenitor cells | pt_BR |
| Type | Article | pt_BR |
| DOI | 10.1038/srep39775 | |
| Abstract | Zika virus (ZIKV) infection has been associated with severe complications both in the developing and adult nervous system. To investigate the deleterious effects of ZIKV infection, we used human neural progenitor cells (NPC), derived from induced pluripotent stem cells (iPSC). We found that NPC are highly susceptible to ZIKV and the infection results in cell death. ZIKV infection led to a marked reduction in cell proliferation, ultrastructural alterations and induction of autophagy. Induction of apoptosis of Sox2(+) cells was demonstrated by activation of caspases 3/7, 8 and 9, and by ultrastructural and flow cytometry analyses. ZIKV-induced death of Sox2(+) cells was prevented by incubation with the pan-caspase inhibitor, Z-VAD-FMK. By confocal microscopy analysis we found an increased number of cells with supernumerary centrosomes. Live imaging showed a significant increase in mitosis abnormalities, including multipolar spindle, chromosome laggards, micronuclei and death of progeny after cell division. FISH analysis for chromosomes 12 and 17 showed increased frequency of aneuploidy, such as monosomy, trisomy and polyploidy. Our study reinforces the link between ZIKV and abnormalities in the developing human brain, including microcephaly. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / São Rafael Hospital. Center for Biotechnology and Cell Therapy. Salvador, BA, Brasil | pt_BR |
| Affilliation | São Rafael Hospital. Center for Biotechnology and Cell Therapy. Salvador, BA, Brasil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
| Affilliation | Federal University of Bahia. Laboratory of Virology, Salvador, BA, Brasil | pt_BR |
| Affilliation | Federal University of Bahia. Laboratory of Virology, Salvador, BA, Brasil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Federal University of Bahia. Department of Pathology. Salvador, BA, Brasil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
| Affilliation | São Rafael Hospital. Center for Biotechnology and Cell Therapy. Salvador, BA, Brasil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / São Rafael Hospital. Center for Biotechnology and Cell Therapy. Salvador, BA, Brasil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / São Rafael Hospital. Center for Biotechnology and Cell Therapy. Salvador, BA, Brasil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / São Rafael Hospital. Center for Biotechnology and Cell Therapy. Salvador, BA, Brasil | pt_BR |
| Affilliation | Hospital Couto Maia. Salvador, BA, Brasil | pt_BR |
| Affilliation | Federal University of Rio de Janeiro. Institute of Biophysics Carlos Chagas Filho. Rio de Janeiro, RJ, Brasil | pt_BR |
| Affilliation | São Rafael Hospital. Center for Biotechnology and Cell Therapy. Salvador, BA, Brasil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / São Rafael Hospital. Center for Biotechnology and Cell Therapy. Salvador, BA, Brasil | pt_BR |
| Subject | Zika Virus | pt_BR |
| Subject | Infection | pt_BR |
| Subject | Brain | pt_BR |
| Subject | Abnormalities nervous system | pt_BR |
| Subject | Humans | pt_BR |
| Subject | Microcephaly | pt_BR |
