| Author | Amaral, Kátia B. | |
| Author | Silva, Thiago P. | |
| Author | Malta, Kássia K. | |
| Author | Carmo, Lívia A. S. | |
| Author | Dias, Felipe F. | |
| Author | Almeida, Mariana E. | |
| Author | Andrade, Gustavo F. S. | |
| Author | Martins, Jefferson S. | |
| Author | Pinho, Roberto R. | |
| Author | Costa Neto, Sócrates F. | |
| Author | Gentile, Rosana | |
| Author | Melo, Rossana C. N. | |
| Access date | 2017-03-09T16:39:25Z | |
| Available date | 2017-03-09T16:39:25Z | |
| Document date | 2016 | |
| Citation | AMARAL, Kátia B. et al. Natural Schistosoma mansoni Infection in the Wild Reservoir Nectomys squamipes Leads to Excessive Lipid Droplet Accumulation in Hepatocytes in the Absence of Liver Functional Impairment. Plos One, v.11, n.11, e0166979, 21p, Nov. 2016. | pt_BR |
| ISSN | 1932-6203 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/17993 | |
| Language | eng | pt_BR |
| Publisher | Public Library of Science | pt_BR |
| Rights | open access | |
| Subject in Portuguese | Schistosoma mansoni | pt_BR |
| Subject in Portuguese | Hepatócitos | pt_BR |
| Subject in Portuguese | Infecção | pt_BR |
| Subject in Portuguese | Gotículas Lipídicas | pt_BR |
| Subject in Portuguese | Rato d`água | pt_BR |
| Title | Natural Schistosoma mansoni Infection in the Wild Reservoir Nectomys squamipes Leads to Excessive Lipid Droplet Accumulation in Hepatocytes in the Absence of Liver Functional Impairment | pt_BR |
| Type | Article | |
| DOI | 10.1371/journal.pone.0166979 | |
| Abstract | Schistosomiasis is a neglected tropical disease of a significant public health impact. The
water rat Nectomys squamipes is one of the most important non-human hosts in the schistosomiasis
mansoni transmission in Brazil, being considered a wild reservoir. Cellular mechanisms
that contribute to the physiological adaptation of this rodent to the Schistosoma
mansoni parasite are poorly understood. Here we identified, for the first time, that a hepatic
steatosis, a condition characterized by excessive lipid accumulation with formation of lipid
droplets (LDs) within hepatocytes, occurs in response to the natural S. mansoni infection of
N. squamipes, captured in an endemic region. Significant increases of LD area in the
hepatic tissue and LD numbers/hepatocyte, detected by quantitative histopathological and
ultrastructural analyses, were paralleled by increased serum profile (total cholesterol and triglycerides)
in infected compared to uninfected animals. Raman spectroscopy showed high
content of polyunsaturated fatty acids (PUFAs) in the liver of both groups. MALDI-TOFF
mass spectroscopy revealed an amplified pool of omega-6 PUFA arachidonic acid in the
liver of infected animals. Assessment of liver functional activity by the levels of hepatic transaminases
(ALT and AST) did not detect any alteration during the natural infection. In summary,
this work demonstrates that the natural infection of the wild reservoir N. squamipes
with S. mansoni elicits hepatic steatosis in the absence of liver functional harm and that
accumulation of lipids, markedly PUFAs, coexists with low occurrence of inflammatory granulomatous
processes, suggesting that lipid stores may be acting as a protective mechanism
for dealing with the infection. | pt_BR |
| Affilliation | Universidade Federal de Juiz de Fora. Departamento de Biologia. Laboratório de Biologia Celular. Juiz de Fora, MG, Brasil. | pt_BR |
| Affilliation | Universidade Federal de Juiz de Fora. Departamento de Biologia. Laboratório de Biologia Celular. Juiz de Fora, MG, Brasil. | pt_BR |
| Affilliation | Universidade Federal de Juiz de Fora. Departamento de Biologia. Laboratório de Biologia Celular. Juiz de Fora, MG, Brasil. | pt_BR |
| Affilliation | Universidade Federal de Juiz de Fora. Departamento de Biologia. Laboratório de Biologia Celular. Juiz de Fora, MG, Brasil. | pt_BR |
| Affilliation | Universidade Federal de Juiz de Fora. Departamento de Biologia. Laboratório de Biologia Celular. Juiz de Fora, MG, Brasil. | pt_BR |
| Affilliation | Universidade Federal de Juiz de Fora. Departamento de Química. Laboratório de Nanoestruturas Plasmônicas, Espectroscopia Molecular e Grupo Estrutura. Juiz de Fora, MG, Brasil. | pt_BR |
| Affilliation | Universidade Federal de Juiz de Fora. Departamento de Química. Laboratório de Nanoestruturas Plasmônicas, Espectroscopia Molecular e Grupo Estrutura. Juiz de Fora, MG, Brasil. | pt_BR |
| Affilliation | Universidade Federal de Juiz de Fora. Departamento de Física; Juiz de Fora, MG, Brasil. | pt_BR |
| Affilliation | Universidade Federal de Juiz de Fora. Departamento de Física; Juiz de Fora, MG, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia e Parasitologia de Mamíferos Silvestres Reservatórios. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia e Parasitologia de Mamíferos Silvestres Reservatórios. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Affilliation | Universidade Federal de Juiz de Fora. Departamento de Biologia. Laboratório de Biologia Celular. Juiz de Fora, MG, Brasil. | pt_BR |
| Subject | Schistosoma mansoni | pt_BR |
| Subject | Infection | pt_BR |
| Subject | Wild Reservoir Nectomys squamipes | pt_BR |
| Subject | lipid droplets | pt_BR |
| Subject | hepatocytes | pt_BR |
