| Author | Pacheco, Paulo Anastácio Furtado | |
| Author | Ferreira, Leonardo Braga Gomes | |
| Author | Mendonça, Leonardo | |
| Author | Ferreira, Dinarte Neto M. | |
| Author | Salles, Juliana Pimenta | |
| Author | Faria, Robson Xavier | |
| Author | Teixeira, Pedro Celso Nogueira | |
| Author | Alves, Luiz Anastácio | |
| Access date | 2017-03-13T14:56:32Z | |
| Available date | 2017-03-13T14:56:32Z | |
| Document date | 2016 | |
| Citation | PACHECO, Paulo Anastácio Furtado; et al. P2X7 receptor as a novel drug delivery system to increase the entrance of hydrophilic drugs into cells during photodynamic therapy. J Bioenerg Biomembr, v.48, p.397–411, 2016. | pt_BR |
| ISSN | 0145-479X | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/18018 | |
| Language | eng | pt_BR |
| Publisher | Springer Verlag (Germany) | pt_BR |
| Rights | restricted access | |
| Subject in Portuguese | Azul de metileno. | pt_BR |
| Subject in Portuguese | Terapia fotodinâmica | pt_BR |
| Subject in Portuguese | Receptor P2X7 | pt_BR |
| Subject in Portuguese | Poro | pt_BR |
| Title | P2X7 receptor as a novel drug delivery system to increase the entrance of hydrophilic drugs into cells during photodynamic therapy | pt_BR |
| Type | Article | |
| DOI | 10.1007/s10863-016-9668-6 | |
| Abstract | The second-generation photosensitizer methylene blue (MB) exhibits photochemical and photophysical properties suitable for photodynamic therapy (PDT)-based cancer treatment. However, the clinical application of MB is limited because of its high hydrophilicity, which hinders its penetration into tumor tissues. Therefore, new methods to improve the entry of MB into the cytoplasm of target cells are necessary. Because MB has a mass of 319 Da, transient pores on the plasma membrane, such as the pore induced by the P2X7 receptor (P2X7R) that allows the passage of molecules up to 900 Da, could be used. Using MTT viability assays, flow cytometry experiments, and fluorescence microscopy, we evaluated the toxicity and phototoxicity of MB and potentiation effects of ATP and MB on cell death processes in the J774 cell line (via a P2X7-associated pore). We observed that treatment with 5 μM MB for 15 min promoted the rate of entry of MB into the cytoplasm to 4.7 %. However, treatment with 5 μM MB and 1 mM ATP for the same amount of time increased this rate to 90.2 %. However, this effect was inhibited by pretreatment with a P2X7 antagonist. We used peritoneal macrophages and a cell line that does not express P2X7R as controls. These cells were more resistant to PDT with MB under the same experimental conditions. Taken together, these results suggest the use of the pore associated with P2X7R as a drug delivery system to increase the passage of hydrophilic drugs into cells that express this receptor, thus facilitating PDT. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Comunicação Celular. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Inflamação. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Comunicação Celular. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Comunicação Celular. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Toxoplasmose. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Toxoplasmose. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Comunicação Celular. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Comunicação Celular. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Subject | Photodynamic therapy | pt_BR |
| Subject | P2X7 receptor | pt_BR |
| Subject | Methylene blue . | pt_BR |
| Subject | Pore | pt_BR |
| e-ISSN | 1573-6881 | |
| Embargo date | 2030-01-01 | |
