| Author | Moura, Tatiana Rodrigues de | |
| Author | Oliveira, Fabiano | |
| Author | Rodrigues, Gabriele C | |
| Author | Carneiro, Marcia Weber | |
| Author | Fukutani, Kiyoshi Ferreira | |
| Author | Novais, Fernanda Oliveira | |
| Author | Miranda, José Carlos | |
| Author | Barral Netto, Manoel | |
| Author | Brodskyn, Claudia Ida | |
| Author | Barral, Aldina Maria Prado | |
| Author | Oliveira, Camila Indiani de | |
| Access date | 2011-03-30T14:23:24Z | |
| Available date | 2011-03-30T14:23:24Z | |
| Document date | 2010 | |
| Citation | MOURA, T. R. de et al. Immunity to Lutzomyia intermedia saliva modulates the inflammatory environment induced by Leishmania braziliensis. PLoS Neglected Tropical Diseases, v.4, n.6, p. e712, 2010. | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/1801 | |
| Language | eng | pt_BR |
| Rights | open access | |
| Title | Immunity to Lutzomyia intermedia saliva modulates the inflammatory environment induced by Leishmania braziliensis | pt_BR |
| Type | Article | pt_BR |
| DOI | 10.1371/journal.pntd.0000712 | |
| Abstract | BACKGROUND: During blood feeding, sand flies inject Leishmania parasites in the presence of saliva. The types and functions of cells present at the first host-parasite contact are critical to the outcome on infection and sand fly saliva has been shown to play an important role in this setting. Herein, we investigated the in vivo chemotactic effects of Lutzomyia intermedia saliva, the vector of Leishmania braziliensis, combined or not with the parasite. METHODS AND FINDINGS: We tested the initial response induced by Lutzomyia intermedia salivary gland sonicate (SGS) in BALB/c mice employing the air pouch model of inflammation. L. intermedia SGS induced a rapid influx of macrophages and neutrophils. In mice that were pre-sensitized with L. intermedia saliva, injection of SGS was associated with increased neutrophil recruitment and a significant up-regulation of CXCL1, CCL2, CCL4 and TNF-alpha expression. Surprisingly, in mice that were pre-exposed to SGS, a combination of SGS and L. braziliensis induced a significant migration of neutrophils and an important modulation in cytokine and chemokine expression as shown by decreased CXCL10 expression and increased IL-10 expression. CONCLUSION: These results confirm that sand fly saliva modulates the initial host response. More importantly, pre-exposure to L. intermedia saliva significantly modifies the host's response to L. braziliensis, in terms of cellular recruitment and expression of cytokines and chemokines. This particular immune modulation may, in turn, favor parasite multiplication. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
| Affilliation | National Institute of Allergy and Infectious Diseases. National Institutes of Health. Vector Molecular Biology Unit. Bethesda, MD, United States of America | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
| DeCS | Saliva/IM | pt_BR |
| DeCS | Psychodidae/IM | pt_BR |
| DeCS | Leishmaniose cutânea/IM | pt_BR |
| DeCS | Leishmania braziliensis/IM | pt_BR |
| DeCS | Inflamação/IM | pt_BR |
| DeCS | Camundongos Endogâmicos BALB C | pt_BR |
