| Author | Ferrão Filho, Aloysio da S. | |
| Author | Soares, Maria Carolina S. | |
| Author | Magalhães, Valéria Freitas de | |
| Author | Azevedo, Sandra M. F. O. | |
| Access date | 2017-03-16T11:48:32Z | |
| Available date | 2017-03-16T11:48:32Z | |
| Document date | 2010 | |
| Citation | FERRÃO FILHO, Aloysio da S. et al. A rapid bioassay for detecting saxitoxins using a Daphnia acute toxicity test. Environmental Pollution, v.158, n.6, p.2084-2093, 2010. | pt_BR |
| ISSN | 0269-7491 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/18053 | |
| Language | eng | pt_BR |
| Publisher | Elsevier | pt_BR |
| Rights | restricted access | |
| Subject in Portuguese | Cianobactérias | pt_BR |
| Subject in Portuguese | Daphnia | pt_BR |
| Subject in Portuguese | Cylindrospermopsis | pt_BR |
| Subject in Portuguese | Cladóceros | pt_BR |
| Subject in Portuguese | Saxitoxinas | pt_BR |
| Title | A rapid bioassay for detecting saxitoxins using a Daphnia acute toxicity test | pt_BR |
| Type | Article | |
| DOI | 10.1016/j.envpol.2010.03.007 | |
| Abstract | Bioassays using Daphnia pulex and Moina micrura were designed to detect cyanobacterial neurotoxins in
raw water samples. Phytoplankton and cyanotoxins from seston were analyzed during 15 months in
a eutrophic reservoir. Effective time to immobilize 50% of the exposed individuals (ET50) was adopted as
the endpoint. Paralysis of swimming movements was observed between w0.5e3 h of exposure to lake
water containing toxic cyanobacteria, followed by an almost complete recovery of the swimming activity
within 24 h after being placed in control water. The same effects were observed in bioassays with
a saxitoxin-producer strain of Cylindrospermopsis raciborskii isolated from the reservoir. Regression
analysis showed significant relationships between ET50 vs. cell density, biomass and saxitoxins content,
suggesting that the paralysis of Daphnia in lake water samples was caused by saxitoxins found in
C. raciborskii. Daphnia bioassay was found to be a sensitive method for detecting fast-acting neurotoxins
in natural samples, with important advantages over mouse bioassays. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Biologia. Laboratório de Avaliação e Promoção da Saúde Ambiental. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Affilliation | Universidade Federal de Juiz de Fora. Faculdade de Engenharia. Departamento de Engenharia Sanitária e Ambiental. Juiz de Fora, MG, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Centro de Ciências da Saúde. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Ecofisiologia e Toxicologia de Cianobactérias. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Centro de Ciências da Saúde. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Ecofisiologia e Toxicologia de Cianobactérias. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Subject | Cyanobacteria | pt_BR |
| Subject | Cladocerans | pt_BR |
| Subject | Daphnia | pt_BR |
| Subject | Saxitoxins | pt_BR |
| Subject | Cylindrospermopsis | pt_BR |
| Embargo date | 2030-01-01 | |
