Author | Ferreira, Paulo Michel Pinheiro | |
Author | Bezerra, Daniel Pereira | |
Author | Silva, Jurandy do Nascimento | |
Author | Costa, Marcília Pinheiro da | |
Author | Ferreira, José Roberto de Oliveira | |
Author | Alencar, Nylane Maria Nunes | |
Author | Figueiredo, Ingrid Samantha Tavares de | |
Author | Cavalheiro, Alberto José | |
Author | Machado, Camila Maria Longo | |
Author | Chammas, Roger | |
Author | Alves, Ana Paula Negreiros Nunes | |
Author | Moraes, Manoel Odorico de | |
Author | Pessoa, Claudia | |
Access date | 2017-03-20T17:15:41Z | |
Available date | 2017-03-20T17:15:41Z | |
Document date | 2016 | |
Citation | FERREIRA, P. M. P. et al. Preclinical anticancer effectiveness of a fraction from Casearia sylvestris and its component Casearin X: in vivo and ex vivo methods and microscopy examinations. Journal of Ethnopharmacology, v. 186, p. 270–279, 2016. | pt_BR |
ISSN | 0378-8741 | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/18081 | |
Sponsorship | Federal Brazilian agency “Conselho Nacional de Desenvolvimento Científico e Tecnológico” (CNPq) for
financial supportin the form of grants (#473167/2012-3) and fellowship (#301976/2013-9) | pt_BR |
Language | eng | pt_BR |
Publisher | Elsevier | pt_BR |
Rights | open access | pt_BR |
Subject in Portuguese | Casearin X | pt_BR |
Subject in Portuguese | Teste de fibra oca | pt_BR |
Subject in Portuguese | Sarcoma 180 | pt_BR |
Subject in Portuguese | Leucograma | pt_BR |
Subject in Portuguese | Antineoplásico | pt_BR |
Title | Preclinical anticancer effectiveness of a fraction from Casearia sylvestris and its component Casearin X: in vivo and ex vivo methods and microscopy examinations | pt_BR |
Type | Article | pt_BR |
DOI | 10.1016/j.jep.2016.04.011 | |
Abstract | Casearia sylvestris (Salicaceae) is found in South America and presents antiulcerogenic, cytotoxic, antimicrobial, anti-inflammatory and antihypertensive activities. Aim ofthestudy: Toassessthe in vivoandexvivo antitumor actionofafractionwithcasearins(FC)andits
main component-CasearinX-isolatedfrom C. sylvestris leaves.
Materials andmethods: Firstly,Sarcoma180bearingSwissmiceweretreatedwithFCandCasXfor
7 days.Secondly,BALB/cnudeanimalsreceivedhollow fibers withcoloncarcinoma(HCT-116)orglio-
blastoma (SF-295)cellsandweretreatedwithFCfor4days.On5thday,proliferationwasdeterminedby
MTT assay.
Results: FC 10and25mg/kg/dayi.p.and50mg/kg/dayoralandCasX25mg/kg/dayi.p.and50mg/kg/
day oralrevealedtumorgrowthinhibitionratesof35.8,86.2,53.7,90.0and65.5%andsuchtumors
demonstratedraremitosesandcoagulationnecrosisareas.Similarly,FCreducedmultiplyingofHCT-116
and SF-295cellswhenevaluatedbytheHollowFiberAssay(2.5and5mg/kg/dayi.p.and25and50mg/
kg/day oral),withcellgrowthinhibitionratesrangingfrom33.3to67.4%(po0.05). Flowcytometry
experimentsrevealedthatFCreducedmembraneintegrityandinducedDNAfragmentationandmi-
tochondrial depolarization(po0.05).
Conclusions: FC andCasXwereefficient antitumorsubstancesagainstmurineandhumancancercells
and causedreversiblemorphologicalchangesinliver,kidneysandspleens,emphasizingclerodanedi-
terpenes asanemergingclassofanticancermolecules. | pt_BR |
Affilliation | Federal University of Piauí. Laboratory in Experimental Cancerology. Department of Biophysics and Physiology.Teresina, PI, Brazil / Federal University of Piauí. Postgraduate Programin Pharmaceutical Sciences. Teresina, PI, Brazil / Federal University of Piauí. Postgraduate Programin in Biotechnology. Teresina, PI, Brazil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
Affilliation | Federal University of Piauí. Postgraduate Programin in Biotechnology. Teresina, PI, Brazil | pt_BR |
Affilliation | Federal University of Piauí. Postgraduate Programin Pharmaceutical Sciences. Teresina, PI, Brazil / Federal University of Piauí. Department of Pharmacy. Teresina, PI, Brazil | pt_BR |
Affilliation | Federal University of Piauí. Postgraduate Programin Pharmaceutical Sciences. Teresina, PI, Brazil | pt_BR |
Affilliation | Federal University of Ceará. Faculty of Medicine. Department of Physiology and Pharmacology. Fortaleza, CE, Brazil | pt_BR |
Affilliation | Federal University of Ceará. Faculty of Medicine. Department of Physiology and Pharmacology. Fortaleza, CE, Brazil | pt_BR |
Affilliation | State University of São Paulo. Chemistry Institute. Araraquara, SP, Brazil | pt_BR |
Affilliation | State University of SãoPaulo. Center for Medical Nuclear. Radioisotopes Research Laboratory. São Paulo, SP, Brazil | pt_BR |
Affilliation | State University of Paulo. São Faculty of Medicine. Department of Radiology. São Paulo, SP, Brazil | pt_BR |
Affilliation | Federal University of Ceará. Department of Clinical Odontology. Fortaleza, CE, Brazil | pt_BR |
Affilliation | Federal University of Ceará. Faculty of Medicine. Department of Physiology and Pharmacology. Fortaleza, CE, Brazil | pt_BR |
Affilliation | Federal University of Ceará. Faculty of Medicine. Department of Physiology and Pharmacology. Fortaleza, CE, Brazil / Oswaldo Cruz Foundation. Fortaleza, CE, Brazil | pt_BR |
Subject | Casearin X | pt_BR |
Subject | Hollow fiber assay | pt_BR |
Subject | Sarcoma 180 | pt_BR |
Subject | Antineoplastic | pt_BR |
Subject | Leukogram | pt_BR |