| Author | Carneiro, Marcia Weber | |
| Author | Fukutani, Kiyoshi Ferreira | |
| Author | Andrade, Bruno de Bezerril | |
| Author | Curvelo, Rebecca Pereira | |
| Author | Cristal, Juqueline Rocha | |
| Author | Carvalho, Augusto Marcelino Pedreira de | |
| Author | Barral, Aldina Maria Prado | |
| Author | Van Weyenbergh, Johan Jozef Rosa Maria | |
| Author | Barral Netto, Manoel | |
| Author | Oliveira, Camila Indiani de | |
| Access date | 2017-03-21T18:00:06Z | |
| Available date | 2017-03-21T18:00:06Z | |
| Document date | 2016 | |
| Citation | Gene Expression Profile of High IFN-γ CARNEIRO, M. W. et al. Producers Stimulated with Leishmania braziliensis Identifies Genes Associated with Cutaneous Leishmaniasis. PLoS Neglected Tropical Diseases, v. 10, n. 11, p. e0005116, 2016. | pt_BR |
| ISSN | 1935-2727 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/18109 | |
| Sponsorship | Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq; grant 302464/2009-3 to M.BN
and fellowship to MWC and KFF), Fundação de Amparo à Pesquisa do Estado da Bahia (FAPESB)
(fellowship to KFF) and Coordenação de Apoio à Pesquisa e Ensino Superior (CAPES) | pt_BR |
| Language | eng | pt_BR |
| Publisher | Public Library of Science | pt_BR |
| Rights | open access | pt_BR |
| Subject in Portuguese | Leishmania braziliensis | pt_BR |
| Subject in Portuguese | Leishmaniose cutânea | pt_BR |
| Subject in Portuguese | Genética | pt_BR |
| Subject in Portuguese | Vacina | pt_BR |
| Subject in Portuguese | Infecção | pt_BR |
| Title | Gene Expression Profile of High IFN-γ Producers Stimulated with Leishmania braziliensis Identifies Genes Associated with Cutaneous Leishmaniasis | pt_BR |
| Type | Article | pt_BR |
| DOI | 10.1371/journal.pntd.0005116 | |
| Abstract | The initial response to Leishmania parasites is essential in determining disease development or resistance. In vitro, a divergent response to Leishmania, characterized by high or low IFN-γ production has been described as a potential tool to predict both vaccine response and disease susceptibility in vivo. Methods and findings
We identified uninfected and healthy individuals that were shown to be either high- or low
IFN-γ producers (HPs and LPs, respectively) following stimulation of peripheral blood cells
with Leishmania braziliensis. Following stimulation, RNA was processed for gene expression
analysis using immune gene arrays. Both HPs and LPs were shown to upregulate the
expression of CXCL10, IFI27, IL6 and LTA. Genes expressed in HPs only (CCL7, IL8,
IFI44L and IL1B) were associated with pathways related to IL17 and TREM 1 signaling. In
LPs, uniquely expressed genes (for example IL9, IFI44, IFIT1 and IL2RA) were associated
with pathways related to pattern recognition receptors and interferon signaling. We then
investigated whether the unique gene expression profiles described here could be recapitulated
in vivo, in individuals with active Cutaneous Leishmaniasis or with subclinical infection.
Indeed, using a set of six genes (TLR2, JAK2, IFI27, IFIT1, IRF1 and IL6) modulated in HPs
and LPs, we could successfully discriminate these two clinical groups. Finally, we demonstrate
that these six genes are significantly overexpressed in CL lesions. Conclusion
Upon interrogation of the peripheral response of naive individuals with diverging IFN-γ production
to L. braziliensis, we identified differences in the innate response to the parasite that
are recapitulated in vivo and that discriminate CL patients from individuals presenting a subclinical
infection | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Fundação Jose Silveira. Multinational Organization Network Sponsoring Translational and Epidemiological Research (MONSTER) Initiative. Salvador, BA, Brasil, | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Federal University of Bahia. School of Medicine. Salvador, BA, Brasil | pt_BR |
| Affilliation | Rega Institute for Medical Research. Department of Microbiology and Immunology. KU Leuven, Leuven, Belgium | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Federal University of Bahia. School of Medicine. Salvador, BA, Brasil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
| Subject | Leishmania braziliensis | pt_BR |
| Subject | Cutaneous leishmaniasis | pt_BR |
| Subject | Genetic | pt_BR |
| Subject | Vaccine | pt_BR |
| xmlui.metadata.dc.subject.ods | 03 Saúde e Bem-Estar | |
