| Author | Rosse, Izinara Cruz | |
| Author | Assis, Juliana Geraldo | |
| Author | Oliveira, Francislon Silva de | |
| Author | Leite, Laura Rabelo | |
| Author | Araújo, Flávio Marcos Gomes | |
| Author | Zerlotini, Adhemar | |
| Author | Volpini, Angela | |
| Author | Dominitini, Anderson Joaquim | |
| Author | Lopes, Beatriz C. | |
| Author | Arbex, Wagner Antonio | |
| Author | Machado, Marco A | |
| Author | Peixoto, Maria G. C. D. | |
| Author | Verneque, Rui S. | |
| Author | Martins, Marta F. | |
| Author | Coimbra, Roney Santos | |
| Author | Silva, Marcos V. G. B. | |
| Author | Oliveira, Guilherme Corrêa de | |
| Author | Carvalho, Maria Raquel S. | |
| Access date | 2017-03-21T18:02:35Z | |
| Available date | 2017-03-21T18:02:35Z | |
| Document date | 2017 | |
| Citation | ROSSE, Izinara Cruz. et al. Whole genome sequencing of Guzera´ cattle reveals genetic variants in candidate genes for production, disease resistance, and heat tolerance. Mamm Genome. V. 28, n. 1-2, p. 66-80, 2017-03-02. doi: 10.1007/s00335-016-9670-7. | pt_BR |
| ISSN | 0938-8990 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/18110 | |
| Language | eng | pt_BR |
| Publisher | Springer | pt_BR |
| Rights | restricted access | pt_BR |
| Subject in Portuguese | mamiferos | pt_BR |
| Subject in Portuguese | genomica | pt_BR |
| Title | Whole genome sequencing of Guzera´ cattle reveals genetic variants in candidate genes for production, disease resistance, and heat tolerance | pt_BR |
| Type | Article | |
| Abstract | n bovines, artificial selection has produced a large number of breeds which differ in production, environmental adaptation, and health characteristics. To investigate the genetic basis of these phenotypical differences, several bovine breeds have been sequenced. Millions of new SNVs were described at every new breed sequenced, suggesting that every breed should be sequenced. Guzerat or Guzerá is an indicine breed resistant to drought and parasites that has been the base for some important breeds such as Brahman. Here, we describe the sequence of the Guzerá genome and the in silico functional analyses of intragenic breed-specific variations. Mate-paired libraries were generated using the ABI SOLiD system. Sequences were mapped to the Bos taurus reference genome (UMD 3.1) and 87% of the reference genome was covered at a 26X. Among the variants identified, 2,676,067 SNVs and 463,158 INDELs were homozygous, not found in any database searched, and may represent true differences between Guzerá and B. taurus. Functional analyses investigated with the NGS-SNP package focused on 1069 new, non-synonymous SNVs, splice-site variants (including acceptor and donor sites, and the conserved regions at both intron borders, referred to here as splice regions) and coding INDELs (NS/SS/I). These NS/SS/I map to 935 genes belonging to cell communication, environmental adaptation, signal transduction, sensory, and immune systems pathways. These pathways have been involved in phenotypes related to health, adaptation to the environment and behavior, and particularly, disease resistance and heat tolerance. Indeed, 105 of these genes are known QTLs for milk, meat and carcass, production, reproduction, and health traits. Therefore, in addition to describing new genetic variants, our approach provided groundwork for unraveling key candidate genes and mutations. | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Instituto de Ciencias Biologicas. Departamento de Biologia Geral. Belo Horizonte, MG, Brazil | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Instituto de Ciencias Biologicas. Departamento de Biologia Geral. Belo Horizonte, MG, Brazil/Fundação Oswaldo Cruz. Centro de Pesquisas Rene Rachou. Grupo de Genomica e Biologia Computacional. Belo Horizonte, MG, Brazil | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Instituto de Ciencias Biologicas. Departamento de Biologia Geral. Belo Horizonte, MG, Brazil/Fundação Oswaldo Cruz. Centro de Pesquisas Rene Rachou. Grupo de Genomica e Biologia Computacional. Belo Horizonte, MG, Brazil | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Instituto de Ciencias Biologicas. Departamento de Biologia Geral. Belo Horizonte, MG, Brazil/Fundação Oswaldo Cruz. Centro de Pesquisas Rene Rachou. Grupo de Genomica e Biologia Computacional. Belo Horizonte, MG, Brazil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Rene Rachou. Grupo de Genomica e Biologia Computacional. Belo Horizonte, MG, Brazil | pt_BR |
| Affilliation | Embrapa Informatica Agropecuaria. Campinas, SP, Brazil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Rene Rachou. Grupo de Genomica e Biologia Computacional. Belo Horizonte, MG, Brazil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Rene Rachou. Grupo de Genomica e Biologia Computacional. Belo Horizonte, MG, Brazil | pt_BR |
| Affilliation | EPAMIG. Belo Horizonte, MG, Brazil | pt_BR |
| Affilliation | Embrapa Gado de Leite. Juiz de Fora, MG, Brazil | pt_BR |
| Affilliation | Embrapa Gado de Leite. Juiz de Fora, MG, Brazil | pt_BR |
| Affilliation | Embrapa Gado de Leite. Juiz de Fora, MG, Brazil | pt_BR |
| Affilliation | Embrapa Gado de Leite. Juiz de Fora, MG, Brazil | pt_BR |
| Affilliation | Embrapa Gado de Leite. Juiz de Fora, MG, Brazil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Rene Rachou. Belo Horizonte, MG, Brazil | pt_BR |
| Affilliation | Embrapa Gado de Leite. Juiz de Fora, MG, Brazil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Rene Rachou. Grupo de Genomica e Biologia Computacional. Belo Horizonte, MG, Brazil/Vale Technology Institute. Belem, PA, Brazil | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Instituto de Ciencias Biologicas. Departamento de Biologia Geral. Belo Horizonte, MG, Brazil | pt_BR |
| Subject | mammalian | pt_BR |
| Subject | genome | pt_BR |
| Embargo date | 2022-01-01 | |
