| Author | Sampaio, Geraldo Pedral | |
| Author | Alves, Jessé dos Santos | |
| Author | Schriefer, Albert | |
| Author | Magalhães, Andréa Santos | |
| Author | Meyer, Roberto | |
| Author | Glesby, Marshall J | |
| Author | Carvalho Filho, Edgar Marcelino | |
| Author | Carvalho, Lucas Pedreira de | |
| Access date | 2017-03-29T16:19:14Z | |
| Available date | 2017-03-29T16:19:14Z | |
| Document date | 2016 | |
| Citation | SAMPAIO, G. P. et al. Detection of IgG Anti-Leishmania Antigen by Flow Cytometry as a Diagnostic Test for. Plos One, v. 11, n. 9, p. e0162793, 2016. | pt_BR |
| ISSN | 1932-6203 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/18195 | |
| Sponsorship | Grant-in-Aid for Scientific Research (C) 24593126 and 16K11836 from the Japan Society for the Promotion
of Science, Tokyo, Japan | pt_BR |
| Language | eng | pt_BR |
| Publisher | Public Library of Science | pt_BR |
| Rights | open access | pt_BR |
| Subject in Portuguese | Leishmania | pt_BR |
| Subject in Portuguese | Citometria de fluxo | pt_BR |
| Subject in Portuguese | Diagnóstico | pt_BR |
| Subject in Portuguese | Leishmaniose cutânea | pt_BR |
| Subject in Portuguese | ELISA | pt_BR |
| Title | Detection of IgG Anti-Leishmania Antigen by Flow Cytometry as a Diagnostic Test for Cutaneous Leishmaniasis | pt_BR |
| Type | Article | pt_BR |
| DOI | 10.1371/journal.pone.0162793 | |
| Abstract | Diagnosis of cutaneous leishmaniasis (CL) relies on clinical presentation, parasite isolation, histopathologic evaluation and positive Montenegro skin test. However, the low amounts of parasites in the lesion of these individuals make parasite isolation and histopatologic diagnosis unreliable, often leading to false-negative results. Also, 15% of people living in endemic areas have sub-clinical infection characterized by positive Montenegro skin test, which may contribute to misdiagnosis. Although the main Leishmania killing mechanism is through cell-mediated immune response, antibodies against Leishmania antigens are found in infected individuals. Here our goal was to develop a new serological technique using polystyrene microspheres sensitized with soluble Leishmania antigens as a tool for the detection of IgG in serum from CL patients by flow cytometry. To validate the assay we carried out a comparative test (ELISA) commonly used as a diagnostic test for parasitic diseases. To determine cross-reactivity we used serum from patients with Chagas disease, caused by a trypanosome that has several proteins with high homology to those of the Leishmania genus. We observed that the flow cytometry technique was more sensitive than the ELISA, but, less specific. Our results show that the flow cytometry serologic test can be used to confirm CL cases in L. braziliensis transmission areas, however, presence of Chagas disease has to be ruled out in these individuals. | pt_BR |
| Affilliation | Universidade Federal da Bahia. Instituto de Ciências da Saúde. Laboratório de Imunologia. Salvador, BA, Brasil | pt_BR |
| Affilliation | Universidade Federal da Bahia. Complexo Hospitalar Universitário Professor Edgard Santos. Serviço de Imunologia. Salvador, BA, Brasil | pt_BR |
| Affilliation | Universidade Federal da Bahia. Complexo Hospitalar Universitário Professor Edgard Santos. Serviço de Imunologia. Salvador, BA, Brasil / Instituto Nacional de Ciências e Tecnologia - Doenças Tropicais. INCT-DT. Salvador, BA, Brasil | pt_BR |
| Affilliation | Universidade Federal da Bahia. Instituto de Ciências da Saúde. Laboratório de Imunologia. Salvador, BA, Brasil | pt_BR |
| Affilliation | Weill Cornell Medical College. Division of Infectious Diseases. New York, USA | pt_BR |
| Affilliation | Universidade Federal da Bahia. Instituto de Ciências da Saúde. Laboratório de Imunologia. Salvador, BA, Brasil | pt_BR |
| Affilliation | Universidade Federal da Bahia. Complexo Hospitalar Universitário Professor Edgard Santos. Serviço de Imunologia. Salvador, BA, Brasil / Instituto Nacional de Ciências e Tecnologia - Doenças Tropicais. INCT-DT. Salvador, BA, Brasil / Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Laboratório Avançado de Saúde Pública. Salvador, BA, Brasil | pt_BR |
| Affilliation | Universidade Federal da Bahia. Complexo Hospitalar Universitário Professor Edgard Santos. Serviço de Imunologia. Salvador, BA, Brasil / Instituto Nacional de Ciências e Tecnologia - Doenças Tropicais. INCT-DT. Salvador, BA, Brasil / Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Laboratório Avançado de Saúde Pública. Salvador, BA, Brasil | pt_BR |
| Subject | Leishmania | pt_BR |
| Subject | Flow Cytometry | pt_BR |
| Subject | Diagnostic | pt_BR |
| Subject | Cutaneous Leishmaniasis | pt_BR |
| Subject | ELISA | pt_BR |
