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AutorSantos, Dilvani Oliveira
AutorCoelho, Janaína Guimarães
AutorNeri, Eloah Christina Lyrio
AutorSouza, Ivy de Castro Campos
AutorPonte, Carlos Germano Garrido
AutorAntas, Paulo Renato Zuquim
Fecha de acceso2017-03-30T16:25:46Z
Fecha de disponibilización2017-03-30T16:25:46Z
Fecha de publicación2016
ReferenciaSANTOS, Dilvani Oliveira; et al. The Differential In Vitro Presentation of Mycobacterium leprae Antigens by Human Dendritic Cells is determined by the Mechanism of Host Cell Adhesion. Journal of Clinical & Cellular Immunology, v.7:4, 8p, Aug. 2016pt_BR
ISSN2155-9899pt_BR
URIhttps://www.arca.fiocruz.br/handle/icict/18220
Idiomaengpt_BR
EditorOMICS Internationalpt_BR
Derechos de autoropen access
Palabras clave en PortuguésHanseníasept_BR
Palabras clave en PortuguésBCGpt_BR
Palabras clave en PortuguésMonócitospt_BR
Palabras clave en PortuguésApresentação do antígenopt_BR
Palabras clave en PortuguésCitocinaspt_BR
Palabras clave en PortuguésAdesão celularpt_BR
TítuloThe Differential In Vitro Presentation of Mycobacterium leprae Antigens by Human Dendritic Cells is determined by the Mechanism of Host Cell Adhesionpt_BR
Tipo del documentoArticle
Resumen en InglésClinical manifestations in leprosy, a chronic infectious disease caused by Mycobacterium leprae, depend on the immunologic reactions of the patient to the bacilli. Previously, we have shown a reversion of the inefficiency of the cellular-mediated immune response against M. leprae in lepromatous leprosy patients when dendritic cells (DCs) were used in vitro as APC. The aim of the current study is to investigate the cell-to-cell interaction when purified human monocytes-derived DCs and macrophages from healthy adult donors were co-cultured with autologous lymphocytes in the presence of either M. leprae or M. bovis BCG by using APCs which were either attached or in suspension. Subsequently, APCs were analyzed by optical microscopy, phenotypically monitored by flow cytometry, and cytokines secretion evaluated by ELISA and Luminex. Overall, our results suggest that: (1) the process of adhesion of the APC may not be absolutely essential for antigen presentation activity; (2) IL-12 production appears to be in direct relation to the susceptibility of the host cell to infection with M. leprae; (3) in the presence of autologous lymphocytes, there is a down-modulation of surface markers studied, except for CD209 in M. leprae-infected CD11c+ DCs, although a similar decreased expression of these markers on macrophages was also observed, and this step occurs in an antigen-dependent manner; and (4) the cytokines secretion is also dependent on the APCs’ adhesion process. In parallel, the human THP-1 cell line was assessed showing that no monokine was differentially regulated when cells remained either attached or in suspension, regardless of the stimulus employed. These results may further guide future pivotal experimental procedures when studying in vitro cellular immune responses against mycobacteria.pt_BR
AfiliaciónUniversidade Federal Fluminense. Depto. de Biologia Celular e Molecular. Laboratório de Biopatógenos e Ativação Celular e Processos Educativos (LaBiopAC&ProEduc). Niterói, RJ, Brasil.pt_BR
AfiliaciónUniversidade Federal Fluminense. Depto. de Biologia Celular e Molecular. Laboratório de Biopatógenos e Ativação Celular e Processos Educativos (LaBiopAC&ProEduc). Niterói, RJ, Brasil.pt_BR
AfiliaciónUniversidade Federal Fluminense. Depto. de Biologia Celular e Molecular. Laboratório de Biopatógenos e Ativação Celular e Processos Educativos (LaBiopAC&ProEduc). Niterói, RJ, Brasil.pt_BR
AfiliaciónUniversidade Federal Fluminense. Depto. de Biologia Celular e Molecular. Laboratório de Biopatógenos e Ativação Celular e Processos Educativos (LaBiopAC&ProEduc). Niterói, RJ, Brasil.pt_BR
AfiliaciónFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunologia Clínica. Rio de Janeiro, RJ. Brasil.pt_BR
AfiliaciónFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunologia Clínica. Rio de Janeiro, RJ. Brasil.pt_BR
Palavras clave en InglêsLeprosypt_BR
Palavras clave en InglêsBCGpt_BR
Palavras clave en InglêsAntigen presentationpt_BR
Palavras clave en InglêsMonocytept_BR
Palavras clave en InglêsCytokinept_BR
Palavras clave en InglêsCell adhesionpt_BR


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