| Author | Miranda, Aracelis | |
| Author | Samudio, Franklyn | |
| Author | González, Kadir | |
| Author | Saldaña, Azael | |
| Author | Brandão, Adeilton | |
| Author | Calzada, Jose E. | |
| Access date | 2017-04-08T14:30:33Z | |
| Available date | 2017-04-08T14:30:33Z | |
| Document date | 2016 | |
| Citation | MIRANDA, Arecelis; et al. Calmodulin Polymerase Chain Reaction–Restriction Fragment Length Polymorphism for Leishmania Identification and Typing. Am. J. Trop. Med. Hyg., v.95, n.2, p.383-387, Aug. 2016. | pt_BR |
| ISSN | 0002-9637 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/18331 | |
| Language | eng | pt_BR |
| Publisher | American Society of Tropical Medicine and Hygiene | pt_BR |
| Rights | restricted access | |
| Subject in Portuguese | Reação em Cadeia da Polimerase | pt_BR |
| Subject in Portuguese | Leishmaniose | pt_BR |
| Subject in Portuguese | Calmodium | pt_BR |
| Title | Calmodulin Polymerase Chain Reaction-Restriction Fragment Length Polymorphism for Leishmania Identification and Typing | pt_BR |
| Type | Article | |
| DOI | 10.4269/ajtmh.15-0709 | |
| Abstract | A precise identification of Leishmania species involved in human infections has epidemiological and clinical importance. Herein, we describe a preliminary validation of a restriction fragment length polymorphism assay, based on the calmodulin intergenic spacer region, as a tool for detecting and typing Leishmania species. After calmodulin amplification, the enzyme HaeIII yielded a clear distinction between reference strains of Leishmania mexicana, Leishmania amazonensis, Leishmania infantum, Leishmania lainsoni, and the rest of the Viannia reference species analyzed. The closely related Viannia species: Leishmania braziliensis, Leishmania panamensis, and Leishmania guyanensis, are separated in a subsequent digestion step with different restriction enzymes. We have developed a more accessible molecular protocol for Leishmania identification/typing based on the exploitation of part of the calmodulin gene. This methodology has the potential to become an additional tool for Leishmania species characterization and taxonomy. | pt_BR |
| Affilliation | Instituto Conmemorativo Gorgas de Estudios de la Salud. Panama City, Panama. | pt_BR |
| Affilliation | Instituto Conmemorativo Gorgas de Estudios de la Salud. Panama City, Panama / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório Interdisciplinar de Pesquisas Médicas. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Instituto Conmemorativo Gorgas de Estudios de la Salud. Panama City, Panama. | pt_BR |
| Affilliation | Instituto Conmemorativo Gorgas de Estudios de la Salud. Panama City, Panama. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório Interdisciplinar de Pesquisas Médicas. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Instituto Conmemorativo Gorgas de Estudios de la Salud. Panama City, Panama. | pt_BR |
| Subject | Polymerase Chain Reaction | pt_BR |
| Subject | Calmodium | pt_BR |
| Subject | Leishmania | pt_BR |
| e-ISSN | 1476-1645 | |
| Embargo date | 2030-01-01 | |
