| Author | Lanzetti, Manuella | |
| Author | Costa, Cristiane Aguiar da | |
| Author | Nesi, Renata Tiscoski | |
| Author | Barroso, Marina Valente | |
| Author | Martins, Vanessa | |
| Author | Victoni, Tatiana | |
| Author | Lagente, Vincent | |
| Author | Pires, Karla Maria Pereira | |
| Author | Silva, Patrícia Machado Rodrigues e | |
| Author | Resende, Angela Castro | |
| Author | Porto, Luis Cristóvão | |
| Author | Benjamim, Cláudia Farias | |
| Author | Valença, Samuel Santos | |
| Access date | 2017-04-11T12:51:25Z | |
| Available date | 2017-04-11T12:51:25Z | |
| Document date | 2012 | |
| Citation | LANZETTI, Manuella; et al. Oxidative stress and nitrosative stress are involved in different stages of proteolytic pulmonary emphysema. Free Radical Biology and Medicine, v.53, p.1993–2001, 2012. | pt_BR |
| ISSN | 0891-5849 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/18366 | |
| Language | eng | pt_BR |
| Publisher | Elsevier | pt_BR |
| Rights | restricted access | |
| Subject in Portuguese | Estresse Oxidativo | pt_BR |
| Subject in Portuguese | Enfisema | pt_BR |
| Subject in Portuguese | Elastase | pt_BR |
| Subject in Portuguese | Radicais Livres | pt_BR |
| Subject in Portuguese | Aminoguanidina | pt_BR |
| Subject in Portuguese | Nitrotirosina | pt_BR |
| Title | Oxidative stress and nitrosative stress are involved in different stages of proteolytic pulmonary emphysema | pt_BR |
| Type | Article | |
| DOI | 10.1016/j.freeradbiomed.2012.09.015 | |
| Abstract | Our aim was to investigate the role of oxidative stress in elastase-induced pulmonary emphysema. C57BL/6 mice were subjected to pancreatic porcine elastase (PPE) instillation (0.05 or 0.5 U per mouse, i.t.) to induce pulmonary emphysema. Lungs were collected on days 7, 14, and 21 after PPE instillation. The control group was sham injected. Also, mice treated with 1% aminoguanidine (AMG) and inducible NO synthase (iNOS) knockout mice received 0.5 U PPE (i.t.), and lungs were analyzed 21 days after. We performed bronchoalveolar lavage, biochemical analyses of oxidative stress, and lung stereology and morphometry assays. Emphysema was observed histologically at 21 days after 0.5 U PPE treatment; tissues from these mice exhibited increased alveolar linear intercept and air-space volume density in comparison with the control group. TNF-α was elevated at 7 and 14 days after 0.5 U PPE treatment, concomitant with a reduction in the IL-10 levels at the same time points. Myeloperoxidase was elevated in all groups treated with 0.5 U PPE. Oxidative stress was observed during early stages of emphysema, with increased nitrite levels and malondialdehyde and superoxide dismutase activity at 7 days after 0.5 U PPE treatment. Glutathione peroxidase activity was increased in all groups treated with 0.5 U PPE. The emphysema was attenuated when iNOS was inhibited using 1% AMG and in iNOS knockout mice. Furthermore, proteolytic stimulation by PPE enhanced the expression of nitrotyrosine and iNOS, whereas the PPE+AMG group showed low expression of iNOS and nitrotyrosine. PPE stimulus also induced endothelial (e) NOS expression, whereas AMG reduced eNOS. Our results suggest that the oxidative and nitrosative stress pathways are triggered by nitric oxide production via iNOS expression in pulmonary emphysema. | pt_BR |
| Affilliation | Universidade do Estado do Rio de Janeiro. Programa de Pós-graduação em Biologia Humana e Experimental. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade do Estado do Rio de Janeiro. Departamento de Farmacologia e Psicobiologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Ciências Biomédicas. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Ciências Biomédicas. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Ciências Biomédicas. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Université de Rennes. INSERM u991. Rennes, France. | pt_BR |
| Affilliation | Université de Rennes. INSERM u991. Rennes, France. | pt_BR |
| Affilliation | Universidade do Estado do Rio de Janeiro. Programa de Pós-graduação em Biologia Humana e Experimental. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Inflamação. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Affilliation | Universidade do Estado do Rio de Janeiro. Departamento de Farmacologia e Psicobiologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade do Estado do Rio de Janeiro. Programa de Pós-graduação em Biologia Humana e Experimental. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade do Estado do Rio de Janeiro. Programa de Pós-graduação em Biologia Humana e Experimental. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade do Estado do Rio de Janeiro. Programa de Pós-graduação em Biologia Humana e Experimental. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Subject | Oxidative stress | pt_BR |
| Subject | Emphysema | pt_BR |
| Subject | Elastase | pt_BR |
| Subject | Aminoguanidine | pt_BR |
| Subject | Nitrotyrosine | pt_BR |
| Subject | Free radicals | pt_BR |
| e-ISSN | 1873-4596) | |
| Embargo date | 2030-01-01 | |
