| Author | Bobosha, Kidist | |
| Author | Tang, Sheila Tuyet | |
| Author | van der Ploeg-van Schip, Jolien J. | |
| Author | Bekele, Yonas | |
| Author | Martins, Marcia V. S. B. | |
| Author | Lind, Ole | |
| Author | Franken, Kees L. M. C. | |
| Author | Khadge, Saraswoti | |
| Author | Pontes, Maria Araci de Andrade | |
| Author | Gonçalves, Heitor de Sá | |
| Author | Hussien, Jemal | |
| Author | Thapa, Pratibha | |
| Author | Kunwar, Chhatra B, | |
| Author | Hagge, Deanna A. | |
| Author | Aseffa, Abraham | |
| Author | Pessolani, Maria Cristina Vidal | |
| Author | Pereira, Geraldo M. B. | |
| Author | Ottenhoff, Tom H. M. | |
| Author | Geluk, Annemieke | |
| Access date | 2017-04-13T18:23:30Z | |
| Available date | 2017-04-13T18:23:30Z | |
| Document date | 2012 | |
| Citation | BOBOSHA, Kidist; et al. Mycobacterium leprae virulence-associated peptides are indicators of exposure to M. leprae in Brazil, Ethiopia and Nepal. Mem Inst Oswaldo Cruz, v.107, Suppl. I, p.112-123, 2012. | pt_BR |
| ISSN | 1678-8080 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/18441 | |
| Language | eng | pt_BR |
| Publisher | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. | pt_BR |
| Rights | open access | |
| Subject in Portuguese | Mycobacterium leprae | pt_BR |
| Subject in Portuguese | Hanseníaae | pt_BR |
| Subject in Portuguese | Biomarcadores | pt_BR |
| Subject in Portuguese | Bioinformática | pt_BR |
| Subject in Portuguese | Virulência | pt_BR |
| Subject in Portuguese | Peptídeos | pt_BR |
| Title | Mycobacterium leprae virulence-associated peptides are indicators of exposure to M. leprae in Brazil, Ethiopia and Nepal | pt_BR |
| Type | Article | |
| Abstract | Silent transmission of Mycobacterium leprae, as evidenced by stable leprosy incidence rates in various countries, remains a health challenge despite the implementation of multidrug therapy worldwide. Therefore, the development of tools for the early diagnosis of M. leprae infection should be emphasised in leprosy research. As part of the continuing effort to identify antigens that have diagnostic potential, unique M. leprae peptides derived from predicted virulenceassociated proteins (group IV.A) were identified using advanced genome pattern programs and bioinformatics. Based on human leukocyte antigen (HLA)-binding motifs, we selected 21 peptides that were predicted to be promiscuous HLA-class I T-cell epitopes and eight peptides that were predicted to be HLA-class II restricted T-cell epitopes for field-testing in Brazil, Ethiopia and Nepal. High levels of interferon (IFN)-γ were induced when peripheral blood mononuclear cells (PBMCs) from tuberculoid/borderline tuberculoid leprosy patients located in Brazil and Ethiopia were stimulated with the ML2055 p35 peptide. PBMCs that were isolated from healthy endemic controls living in areas with high leprosy prevalence (EChigh) in Ethiopia also responded to the ML2055 p35 peptide. The Brazilian EChigh group recognised the ML1358 p20 and ML1358 p24 peptides. None of the peptides were recognised by PBMCs from healthy controls living in non-endemic region. In Nepal, mixtures of these peptides induced the production of IFN-γ by the PBMCs of leprosy patients and EChigh. Therefore, the M. leprae virulence-associated peptides identified in this study may be useful for identifying exposure to M. leprae in population with differing HLA polymorphisms.
Key | pt_BR |
| Affilliation | University Medical Center. Department of Infectious Diseases. Leiden, The Netherlands / Armauer Hansen Research Institute, Addis Ababa, Ethiopia. | pt_BR |
| Affilliation | University Denmark. Center for Biological Sequence Analysis. Lyngby, Denmark. | pt_BR |
| Affilliation | University Medical Center. Department of Infectious Diseases. Leiden, The Netherlands. | pt_BR |
| Affilliation | Armauer Hansen Research Institute, Addis Ababa, Ethiopia. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Microbiologia Celular. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | University Denmark. Center for Biological Sequence Analysis. Lyngby, Denmark. | pt_BR |
| Affilliation | University Medical Center. Department of Infectious Diseases. Leiden, The Netherlands. | pt_BR |
| Affilliation | Anandaban Hospital. Mycobacterial Research Laboratory. Kathmandu, Nepal. | pt_BR |
| Affilliation | Centro Dermatológico Dona Libânia. Fortaleza, CE, Brasil. | pt_BR |
| Affilliation | Centro Dermatológico Dona Libânia. Fortaleza, CE, Brasil. | pt_BR |
| Affilliation | Armauer Hansen Research Institute, Addis Ababa, Ethiopia. | pt_BR |
| Affilliation | Anandaban Hospital. Mycobacterial Research Laboratory. Kathmandu, Nepal. | pt_BR |
| Affilliation | Anandaban Hospital. Mycobacterial Research Laboratory. Kathmandu, Nepal. | pt_BR |
| Affilliation | Anandaban Hospital. Mycobacterial Research Laboratory. Kathmandu, Nepal. | pt_BR |
| Affilliation | Armauer Hansen Research Institute, Addis Ababa, Ethiopia. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Microbiologia Celular. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade do Estado do Rio de Janeiro. Faculdade de Ciências Médicas. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | University Medical Center. Department of Infectious Diseases. Leiden, The Netherlands. | pt_BR |
| Subject | Mycobacterium leprae | pt_BR |
| Subject | Leprosy | pt_BR |
| Subject | biomarkers | pt_BR |
| Subject | bioinformatics | pt_BR |
| Subject | virulence | pt_BR |
| Subject | peptides | pt_BR |
