| Author | Orjuela-Sánchez, Pamela | |
| Author | Duggan, Erika | |
| Author | Nolan, John | |
| Author | Frangos, John A. | |
| Author | Carvalho, Leonardo J. M. | |
| Access date | 2017-04-18T16:07:33Z | |
| Available date | 2017-04-18T16:07:33Z | |
| Document date | 2012 | |
| Citation | ORJUELA-SANCHEZ, Pamela; et al. A lactate dehydrogenase ELISA-based assay for the in vitro determination of Plasmodium berghei sensitivity to anti-malarial drugs. Malaria Journal, v.11:366, 8p, Nov. 2012. | pt_BR |
| ISSN | 1475-287 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/18497 | |
| Language | eng | pt_BR |
| Publisher | BioMed Central | pt_BR |
| Rights | open access | |
| Subject in Portuguese | Plasmodium berghei | pt_BR |
| Subject in Portuguese | Cultura in vitro | pt_BR |
| Subject in Portuguese | Infecção sincronizada | pt_BR |
| Subject in Portuguese | Ensaio in vitro com fármaco | pt_BR |
| Subject in Portuguese | Vacinas Antimaláricas | pt_BR |
| Subject in Portuguese | L-Lactato Desidrogenase | pt_BR |
| Subject in Portuguese | Ensaio de Imunoadsorção Enzimática | pt_BR |
| Title | A lactate dehydrogenase ELISA-based assay for the in vitro determination of Plasmodium berghei sensitivity to anti-malarial drugs | pt_BR |
| Type | Article | |
| DOI | 10.1186/1475-2875-11-366 | |
| Abstract | Plasmodium berghei rodent malaria is a well-known model for the investigation of anti-malarial drug efficacy in vivo. However, the availability of drug in vitro assays in P. berghei is reduced when compared with the spectrum of techniques existing for Plasmodium falciparum. New alternatives to the current manual or automated methods described for P. berghei are attractive. The present study reports a new ELISA drug in vitro assay for P. berghei using two monoclonal antibodies against the parasite lactate dehydrogenase (pLDH). | pt_BR |
| Affilliation | La Jolla Bioengineering Institute. San Diego, CA, USA. | pt_BR |
| Affilliation | La Jolla Bioengineering Institute. San Diego, CA, USA. | pt_BR |
| Affilliation | La Jolla Bioengineering Institute. San Diego, CA, USA. | pt_BR |
| Affilliation | La Jolla Bioengineering Institute. San Diego, CA, USA. | pt_BR |
| Affilliation | La Jolla Bioengineering Institute. San Diego, CA, USA / Fundação Oswaldo Cruz. Instituto Oswaldo cruz. Laboratório de Pesquisa em Malária. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Subject | In vitro culture | pt_BR |
| Subject | schizont | pt_BR |
| Subject | synchronized infection | pt_BR |
| Subject | drug in vitro assay | pt_BR |
| Subject | anti-malarials | pt_BR |
| Subject | IC50 values | pt_BR |
| Subject | lactate dehydrogenase | pt_BR |
| Subject | ELISA | pt_BR |
| Subject | Plasmodium berghei | pt_BR |
| xmlui.metadata.dc.subject.ods | 03 Saúde e Bem-Estar | |
