| Author | Caldeira, Roberta Lima | |
| Author | Teodoro, Tatiana Maria | |
| Author | Jannotti-Passos, Liana Konovaloff | |
| Author | Lira-Moreira, Pollanah M. | |
| Author | Goveia, Christiane de Oliveira | |
| Author | Carvalho, Omar dos Santos | |
| Access date | 2017-04-26T15:22:46Z | |
| Available date | 2017-04-26T15:22:46Z | |
| Document date | 2016 | |
| Citation | CALDEIRA, R. L. et al. Characterization of South American Snails of the Genus Biomphalaria (Basommatophora: Planorbidae) and Schistosoma mansoni (Platyhelminthes: Trematoda) in Molluscs by PCR-RFLP. BioMed Research International, v. 2016, p. 1–5, 2016. | |
| ISSN | 2314-6141 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/18666 | |
| Sponsorship | Este trabalho foi parcialmente financiado por doações da FAPEMIG;
CNPq (304121 / 2014-2); e Fiocruz. | |
| Language | eng | pt_BR |
| Rights | open access | pt_BR |
| Subject in Portuguese | PCR | |
| Subject in Portuguese | Schistosoma mansoni | |
| Subject in Portuguese | Caramujos | |
| Title | Characterization of South American Snails of the Genus Biomphalaria (Basommatophora: Planorbidae) and Schistosoma mansoni (Platyhelminthes: Trematoda) in Molluscs by PCR-RFLP | pt_BR |
| Type | Article | pt_BR |
| DOI | 10.1155/2016/1045391 | pt_BR |
| Abstract | The identification of snails of the genus Biomphalaria can be done using morphological characteristics which depends on the size of the snails and skill and knowledge of researcher. These methods sometimes are not adequate for identification of species. The PCR-RFLP, using the ITS region of the rDNA, has been used to identify Brazilian species of the genus Biomphalaria. Nevertheless, there is a lack of information about snails from other Latin American countries. In addition, some snails may be infected by Schistosoma mansoni and when submitted to PCR-RFLP they show molecular profiles different from those previously standardized for the other mollusc species. In this work the molecular profiles of 15 species and the subspecies were established by PCR-RFLP of ITS-rDNA with the enzyme DdeI. Moreover, the molecular profiles of host species, B. glabrata, B. straminea, B. tenagophila, and B. prona, infected by S. mansoni were also established. The molluscs were dissected to permit morphological identification. These results contribute to a correct identification of snails of the genus Biomphalaria and detection of these snails infected by S. mansoni. | |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Grupo de Pesquisa em Helmintologia e Malacologia Médica. Belo Horizonte, MG, Brasil. | |
| Affilliation | Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Entomologia. Biociencias e Biotecnologia em Saúde. Recife, PE, Brasil. | |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Moluscario “Lobato Paraense”. Belo Horizonte, MG, Brasil. | |
| Affilliation | Prefeitura Municipal de Belo Horizonte. Belo Horizonte, MG, Brasil. | |
| Affilliation | Instituto Evandro Chagas. Sessão de Parasitologia. Laboratório de Parasitoses Intestinais e Malacologia. Ananindeua, PA, Brasil. | |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Grupo de Pesquisa em Helmintologia e Malacologia Médica. Belo Horizonte, MG, Brasil. | |
| DeCS | Biomphalaria / parasitologia | |
| DeCS | DNA, Helmintos / genética | |
| DeCS | DNA ribossômico / genética | |
| DeCS | Polimorfismo, comprimento do fragmento de restrição | |
| DeCS | Schistosoma mansoni / genética | |
