| Author | Lima, Lyana Rodrigues Pinto | |
| Author | Silva, Amanda Perse da | |
| Author | Schmidt-Chanasit, Jonas | |
| Author | Paula, Vanessa Salete de | |
| Access date | 2017-05-04T12:26:52Z | |
| Available date | 2017-05-04T12:26:52Z | |
| Document date | 2017 | |
| Citation | LIMA, Lyana Rodrigues Pinto; et al. Diagnosis of human herpes virus 1 and 2 (HHV-1 and HHV-2): use of a synthetic standard curve for absolute quantification by real time polymerase chain reaction. Mem Inst Oswaldo Cruz, v.112, n.3, p.220-223, Mar. 2017. | pt_BR |
| ISSN | 0074-0276 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/18729 | |
| Language | eng | pt_BR |
| Publisher | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz | pt_BR |
| Rights | open access | |
| Subject in Portuguese | Reação em cadeia da polimerase em tempo real | pt_BR |
| Subject in Portuguese | Curva sintética | pt_BR |
| Subject in Portuguese | Herpes | pt_BR |
| Title | Diagnosis of human herpes virus 1 and 2 (HHV-1 and HHV-2): use of a synthetic standard curve for absolute quantification by real time polymerase chain reaction | pt_BR |
| Type | Article | |
| DOI | 10.1590/0074-02760160354 | |
| Abstract | The use of quantitative real time polymerase chain reaction (qPCR) for herpesvirus detection has improved the sensitivity and specificity of diagnosis, as it is able to detect shedding episodes in the absence of clinical lesions and diagnose clinical specimens that have low viral loads. With an aim to improve the detection and quantification of herpesvirus by qPCR, synthetic standard curves for human herpesvirus 1 and 2 (HHV-1 and HHV-2) targeting regions gD and gG, respectively, were designed and evaluated. The results show that synthetic curves can replace DNA standard curves in diagnostic herpes qPCR. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Desenvolvimento Tecnológico em Virologia. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Desenvolvimento Tecnológico em Virologia. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Affilliation | Bernhard Nocht Institute for Tropical Medicine. Diagnostic Virology Laboratory. Hamburg, Germany. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Desenvolvimento Tecnológico em Virologia. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Subject | real time PCR | pt_BR |
| Subject | synthetic curve | pt_BR |
| Subject | herpes | pt_BR |
| e-ISSN | 1678-8060 | |
