Author | Marins, Rachel Siqueira de Queiroz Simões | |
Author | Cassiano, Keila Mara | |
Author | Pereira, Sílvia Regina Ferreira Gonçalves | |
Author | Nogueira, Denise Monnerat | |
Author | Carvalho, Eulógio Queiroz de | |
Access date | 2017-05-11T13:59:01Z | |
Available date | 2017-05-11T13:59:01Z | |
Document date | 2012 | |
Citation | MARINS, Rachel Siqueira de Queiroz Simões; et al. Canine latent papillomavirus infection and chromosomal instability studies in peripheral blood lymphocytes and tumors cells cultures from lesions biopsy. International Research Journal of Biochemistry and Bioinformatics, v. 2, n.3, p.62-68, Mar. 2012. | pt_BR |
ISSN | 2250-9941 | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/18816 | |
Language | eng | pt_BR |
Publisher | International Research Journals | pt_BR |
Rights | open access | pt_BR |
Subject in Portuguese | Papilomavírus canino | pt_BR |
Subject in Portuguese | Culturas de linfócitos do sangue periférico | pt_BR |
Subject in Portuguese | Culturas celulares | pt_BR |
Subject in Portuguese | Reação em Cadeia da Polimerase | pt_BR |
Title | Canine latent papillomavirus infection and chromosomal instability studies in peripheral blood lymphocytes and tumors cells cultures from lesions biopsy | pt_BR |
Type | Article | pt_BR |
Abstract | In the present study, we reported about papillomavirus (PV) clinical infection and viral latent
investigation in canines. Clinical and molecular analyses for detection of DNA PV infection, as well
as histopathology examination and cytogenetic evaluation were carried out in clinical lesions
canines. Blood sample from the both epithelial infection and the asymptomatic dogs had been
collected for molecular analysis. Amplification of DNA samples with the L1 gene from generic
primers were detected in skin biopsies and blood samples from canines healthy clinically or not. To
investigate the chromosomal fragility, we researched by cytogenetic technique in the peripheral
blood lymphocytes cultures and in the tumors cells cultures from canines presenting oral and
cutaneous warts lesions. The histological examinations of the skin biopsy specimens were
diagnosed like adenoma, carcinoma and papilloma. In some cases, besides the surgical removal,
autogenous vaccine derived from fresh warts of the same animal was indicated. The findings
documented as mucosal and cutaneous tropism, which the PV viral infection not confined only
epithelial sites, but also involved intracellular genome of host cell. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Molecular. Rio de Janeiro, RJ. Brasil. | pt_BR |
Affilliation | Universidade Federal Fluminense. Instituto de Matemática. Laboratório de Estatística. Campus Valonguinho, Niterói, RJ, Brasil. | pt_BR |
Affilliation | Universidade Estadual do Norte Fluminense Darcy Ribeiro. Centro de Agricultura e Tecnologia Agrícola. Campos dos Goytacazes, RJ, Brasil. | pt_BR |
Affilliation | Universidade Federal Fluminense. Faculdade de Veterinária. Laboratório de Citogenética Animal. Campus Vital Brasil. Niterói, RJ, Brasil. | pt_BR |
Affilliation | Universidade Estadual do Norte Fluminense Darcy Ribeiro. Centro de Agricultura e Tecnologia Agrícola. Departamento de Anatomia Patológica e Morfológica. Laboratório de Saúde Animal. Campos dos Goytacazes, RJ, Brasil | pt_BR |
Subject | canine papillomavirus | pt_BR |
Subject | peripheral blood lymphocytes cultures | pt_BR |
Subject | tissue cell cultures | pt_BR |
Subject | PCR | pt_BR |