| Author | Matsuda, Monique | pt_BR |
| Author | Krempel, Paloma Gava | pt_BR |
| Author | André, P. A | pt_BR |
| Author | Freitas, J. R | pt_BR |
| Author | Freitas, Luiz Antonio Rodrigues de | pt_BR |
| Author | Veras, Patrícia Sampaio Tavares | pt_BR |
| Author | Saldiva, P. H. N | pt_BR |
| Author | Marquezini, Monica Valeria | pt_BR |
| Access date | 2017-05-29T16:20:38Z | |
| Available date | 2017-05-29T16:20:38Z | |
| Document date | 2013 | |
| Citation | MATSUDA, Monique et al. Traffic-related air pollution effect on fast glycemia of aged obese type 2 diabetic mice. Journal of Clinical & Experimental Cardiology, v. 4, n. 7, p. 1-5, 2013. | pt_BR |
| ISSN | 2155-9880 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/19035 | |
| Sponsorship | Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Grant 2008/57717-6 and Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) grant 2008/57717-6. | pt_BR |
| Language | eng | pt_BR |
| Publisher | OMICS International | pt_BR |
| Rights | open access | pt_BR |
| Subject in Portuguese | Idoso | pt_BR |
| Subject in Portuguese | Obesidade | pt_BR |
| Subject in Portuguese | Ratos diabéticos Tipo 2 | pt_BR |
| Subject in Portuguese | Poluição do ar | pt_BR |
| Title | Traffic-related air pollution effect on fast glycemia of aged obese type 2 diabetic mice | en |
| Type | Article | pt_BR |
| DOI | 10.4172/2155-9880.1000255 | pt_BR |
| Abstract | Recent experimental data have provided associations between ambient PM2.5 (fine particulate matter; diameter
≤ 2.5 μm) and propensity to inflammation and chronic diseases especially among susceptible groups, such as
elderly people. There is cumulative evidence that type-2 diabetes mellitus is a chronic inflammatory state aggravated
by factors that promote endothelium inflammation. Accordingly our hypothesis that the exposure of aged obese
population to PM2.5 might aggravate type-2 diabetes, we used a model of aged, diet-induced obese mice. C57BL6
male mice were fed with regular chow (n=30; RC) or high-fat chow (n=36; HF) during one-year and randomly
assigned to filtered (FA-RC, n=16; FA-HF, n=19) or PM2.5 concentrated air (600 μg.m-3) (EXP-RC, n=14; EXPHF,
n=17) chambers to have a daily 1 hour exposition during consecutive 30- days. Fast glycemia was measured
before the animals were euthanized. The Institution’s Ethics Committee approved all experimental procedures.
Heart mRNA content of selected migration, signalization and adhesion proteins were measured by SYBR Green
fluorescence Real Time RT-PCR protocol using appropriate primers. There were no difference between RC-EXP
and RC-FA nor between HF-EXP and HF-FA body weight. Regarding fast glycemia, both, RC and HF groups, were
diabetic, but only the HF group was affected by acute exposure to PM2.5 (mean ± SD, EXP-HF vs FA- HF, 172.8 ±
23.4 vs 156.7 ± 17.6, p <0.05; EXP-RC vs FA-RC, 149.8 ± 19.2, 139.7 ± 15.3, ns; ANOVA). The gene expression
profile of E-selectin, IL-6, VCAM-1, ICAM-1 and MMP-9, was differently affected by PM2.5 in heart and lung. Proteins
activated by inflammatory stimuli involved in the inhibition of insulin signaling are being investigated. | pt_BR |
| Affilliation | University of São Paulo Medical School. Laboratory of Investigation in Ophthalmology. São Paulo, SP, Brasi | pt_BR |
| Affilliation | University of São Paulo Medical School. Experimental Air Pollution Laboratory of Pathology Department. São Paulo, SP, Brasil | pt_BR |
| Affilliation | Federal University of Bahia. Medical School. Department of Pathology and Forensic Medicine. Salvador, BA, Brazil | pt_BR |
| Affilliation | Federal University of Bahia. Medical School. Department of Pathology and Forensic Medicine. Salvador, BA, Brazil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Salvador, BA, Brasil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Salvador, BA, Brasil | pt_BR |
| Affilliation | University of São Paulo Medical School. Experimental Air Pollution Laboratory of Pathology Department. São Paulo, SP, Brasil | pt_BR |
| Affilliation | University of São Paulo Medical School. Experimental Air Pollution Laboratory of Pathology Department. São Paulo, SP, Brasil / Pro-Sangue Foundation. São Paulo, SP, Brasil | pt_BR |
| Subject | Aged | pt_BR |
| Subject | Obesity | pt_BR |
| Subject | Type 2 diabetic mice | pt_BR |
| Subject | Air pollution | pt_BR |
| DeCS | Idoso | pt_BR |
| DeCS | Obesidade | pt_BR |
| DeCS | Poluição do ar | pt_BR |
| DeCS | Glicemia | pt_BR |
| xmlui.metadata.dc.subject.ods | 03 Saúde e Bem-Estar | |
