| Author | Carrara, Vanessa da Silva | |
| Author | Serra, Lara Zampar | |
| Author | Cardozo Filho, Lúcio | |
| Author | Cunha, Edézio F. | |
| Author | Santos, Eduardo C. Torres | |
| Author | Cortez, Diógenes Aparício Garcia | |
| Access date | 2017-05-30T14:45:20Z | |
| Available date | 2017-05-30T14:45:20Z | |
| Document date | 2011 | |
| Citation | CARRARA, Vanessa da Silva; et al. HPLC Analysis of Supercritical Carbon Dioxide and Compressed Propane Extracts from Piper amalago L. with Antileishmanial Activity. Molecules, v.17, p.15-33, 2012. | pt_BR |
| ISSN | 1420-3049 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/19063 | |
| Language | eng | pt_BR |
| Publisher | MDPI | pt_BR |
| Rights | open access | |
| Subject in Portuguese | Dióxido de carbono supercrítico | pt_BR |
| Subject in Portuguese | Alcaloide de pirrolidina | pt_BR |
| Subject in Portuguese | Atividade antileishmanial | pt_BR |
| Subject in Portuguese | Leishmaniose | pt_BR |
| Title | HPLC analysis of supercritical carbon dioxide and compressed propane extracts from Piper amalago L. with antileishmanial activity | pt_BR |
| Type | Article | |
| DOI | 10.3390/molecules17010015 | |
| Abstract | Piper amalago L. leaves were extracted with supercritical carbon dioxide and compressed propane under different conditions, and with chloroform by the conventional maceration method. These methods were compared for the pyrrolidine alkaloid content. Supercritical carbon dioxide (SFE-CO₂) at 313 K and 12.55 MPa showed the highest selectivity for the main compound (600.53 mg/g of extract). A gradient high-performance liquid chromatography (HPLC) method was developed and validated to quantify the alkaloid N-[7-(3',4'-methylenedioxyphenyl)-2(Z),4(Z)-heptadienoyl]pyrrolidine in the extracts. The HPLC method showed linearity, precision and accuracy, allowing the quantitative analysis of the alkaloid in all the samples. All the extracts were tested against the promastigote and intracellular amastigote forms of Leishmania amazonensis. The antileishmanial activity was evaluated in terms of inhibitory concentration for 50% of protozoa (IC₅₀). The cytotoxicity was also evaluated against J774A1 macrophages, and the cytotoxic concentrations for 50% of macrophages were obtained (CC₅₀). The SFE-CO₂ (313 K; 12.55 MPa) extract showed the highest antileishmanial activity with the following IC₅₀ values of 16 and 7 µg/mL against the promastigotes and intracellular amastigotes forms, respectively. The extract showed low cytotoxicity with a CC₅₀ value of 93 µg/mL. | pt_BR |
| Affilliation | Universidade Estadual de Maringá. Departamento de Farmácia. Maringá, PR, Brasil. | pt_BR |
| Affilliation | Universidade Estadual de Maringá. Departamento de Farmácia. Maringá, PR, Brasil. | pt_BR |
| Affilliation | Universidade Estadual de Maringá. Departamento de Engenharia Química. Maringá, PR, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Bioquímica de Tripanossomatídeos. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Bioquímica de Tripanossomatídeos. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Affilliation | Universidade Estadual de Maringá. Departamento de Farmácia. Maringá, PR, Brasil. | pt_BR |
| Subject | Piper amalago L. | pt_BR |
| Subject | pyrrolidine alkaloid | pt_BR |
| Subject | supercritical carbon dioxide | pt_BR |
| Subject | HPLC validation | pt_BR |
| Subject | antileishmanial activity | pt_BR |
| Subject | Leishmaniasis | pt_BR |
| e-ISSN | 1420-3049 | |
| xmlui.metadata.dc.subject.ods | 13 Ação contra a mudança global do clima | |
