| Author | Lemos, Camila Souza | |
| Author | Campos, Simone Neves de | |
| Author | Teva, Antonio | |
| Author | Porrozzi, Renato | |
| Author | Grimaldi Junior, Gabriel | |
| Access date | 2017-06-01T17:09:02Z | |
| Available date | 2017-06-01T17:09:02Z | |
| Document date | 2011 | |
| Citation | LEMOS, Camila Souza; et al. In situ characterization of the granulomatous immune response with time in nonhealing lesional skin of Leishmania braziliensis-infected rhesus macaques (Macaca mulatta). Veterinary Immunology and Immunopathology, v.142, p.147– 155, 2011. | pt_BR |
| ISSN | 0165-2427 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/19089 | |
| Language | eng | pt_BR |
| Publisher | Elsevier | pt_BR |
| Rights | restricted access | |
| Subject in Portuguese | Leishmaniose | pt_BR |
| Subject in Portuguese | Infecção cutãnea | pt_BR |
| Subject in Portuguese | Histopatologia | pt_BR |
| Subject in Portuguese | População celular em lesão | pt_BR |
| Title | In situ characterization of the granulomatous immune response with time in nonhealing lesional skin of Leishmania braziliensis-infected rhesus macaques (Macaca mulatta) | pt_BR |
| Type | Article | |
| DOI | 10.1016/j.vetimm.2011.05.002 | |
| Abstract | We have recently introduced a macaque (Macaca mulatta) model of Leishmania braziliensis-induced self-healing cutaneous leishmaniasis in which the T cell-mediated inflammatory response effectively promotes parasite clearance and granuloma resolution. Here we show that macaques infected with a highly pathogenic L. braziliensis strain displayed longstanding granulomatous lesions which lasted until the end of the observation period (52 weeks). Immunoperoxidase staining of representative tissue sections indicated that distinct cell populations (CD3, CD4, CD8, CD20, Foxp3, CD20, CD68, HLA-DR, CCL2, and CXCL-10) change uniformly during infection, suggesting that the same components of the local immune response are working in unison. This model also confirmed that granuloma formation is orchestrated by diverse inflammatory mediators that are important for T helper type 1 (Th1) cell development and macrophage effector functions. Cytometry analysis of ex vivo granuloma-derived leukocytes revealed accumulation of distinct functional subsets of effector and regulatory T cells into the inflamed skin. We provide evidence that local interleukin (IL)-10 production by both Foxp3(+) and Foxp3(-) CD4(+) T subsets is likely important in promoting lesional granuloma maintenance. Further studying the immune suppression mechanisms that induces granulomas in L. braziliensis-infected macaques may reveal new opportunities for therapeutic control of this important human disease. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Leishmaniose. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Leishmaniose. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Leishmaniose. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Leishmaniose. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Leishmaniose. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Subject | Macaque (Macaca mulatta) model | pt_BR |
| Subject | Leishmania braziliensis cutaneous infection | pt_BR |
| Subject | Histopathology | pt_BR |
| Subject | Cell populations in lesion | pt_BR |
| e-ISSN | 1873-2534 | |
| Embargo date | 2030-01-01 | |
