Author | Rodriguez-Bonfante, Claudina | |
Author | Bonfante-Garrido, Rafael | |
Author | Grimaldi Jr., Gabriel | |
Author | Momen, Hooman | |
Author | Cupolillo, Elisa | |
Access date | 2017-06-06T16:35:06Z | |
Available date | 2017-06-06T16:35:06Z | |
Document date | 2003 | |
Citation | RODRIGUEZ-BONFANTE, Claudina; et al. Genotypically distinct Leishmania colombiensis isolates from Venezuela cause both cutaneous and visceral leishmaniasis in humans. Infection, Genetics and Evolution, v.3, n.2, p.119–124, July 2003. | pt_BR |
ISSN | 1567-1348 | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/19326 | |
Language | eng | pt_BR |
Publisher | Elsevier | pt_BR |
Rights | restricted access | pt_BR |
Subject in Portuguese | Leismaniose visceral | pt_BR |
Subject in Portuguese | Leishmaniose cutânea | pt_BR |
Subject in Portuguese | Reação em Cadeia da Polimerase | pt_BR |
Subject in Portuguese | Técnica de Amplificação ao Acaso de DNA Polimórfico | pt_BR |
Subject in Portuguese | Genotipagem | pt_BR |
Title | Genotypically distinct Leishmania colombiensis isolates from Venezuela cause both cutaneous and visceral leishmaniasis in humans | pt_BR |
Type | Article | pt_BR |
DOI | 10.1016/S1567-1348(03)00012-1 | |
Abstract | Cutaneous leishmaniasis in Venezuela has been associated with different Leishmania species and the visceral form is frequently related
to L. chagasi. However, in this study we have characterized as L. colombiensis by molecular techniques some isolates of Leishmania from
patients presenting either cutaneous or visceral leishmaniasis. Three different molecular markers (two DNA and one protein) were used
to investigate the diversity among the L. colombiensis isolates. The analysis revealed the isolates to be similar although distinct from the
reference strain of L. colombiensis. Slight variation among the isolates could not be correlated with any epidemiological features of the
samples however the visceral isolates were observed to cluster on a phenetic tree derived from one DNA marker. The results confirm
previous findings that L. colombiensis can cause both the cutaneous and visceral forms of the disease and that there exists at least two
distinct etiological agents of American visceral leishmaniasis (AVL). The report also shows the necessity of careful differentiation of
leishmanial isolates in any clinical or epidemiological study of human leishmaniasis. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento Bioquímica e Biologia Molecular. Rio de Janeiro, RJ. Brasil / Universidad Centro-Ocidental Lisandro Alvarado. Barquisimeto, Lara, Venezuela. | pt_BR |
Affilliation | Universidad Centro-Ocidental Lisandro Alvarado. Barquisimeto, Lara, Venezuela. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Imunologia. Laboratório de Pesquisas em Leishmanioses. Rio de Janeiro, RJ. Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento Bioquímica e Biologia Molecular. Rio de Janeiro, RJ. Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Imunologia. Laboratório de Pesquisas em Leishmanioses. Rio de Janeiro, RJ. Brasil. | pt_BR |
Subject | Leishmania colombiensis | pt_BR |
Subject | Cutaneous leishmaniasis | pt_BR |
Subject | Visceral leishmaniasis | pt_BR |
Subject | MLEE | pt_BR |
Subject | RAPD | pt_BR |
Subject | PCR-ITSrDNA | pt_BR |
Subject | Genotyping | pt_BR |
e-ISSN | 1567-7257 | |