| Autor | Schenkman, Sergio | |
| Autor | Pontes-de-Carvalho, Lain Carlos | |
| Autor | Nussenzweig, Victor | |
| Data de acesso | 2017-06-21T18:15:24Z | |
| Data de disponibilização | 2017-06-21T18:15:24Z | |
| Data do publicação | 1992 | |
| Citação | SCHENKMAN, S.; PONTES-DE-CARVALHO, L. C.; NUSSENZWEIG, V. Trypanosoma cruzi Trans-sialidase and Neuraminidase Activities Can Be Mediated by the Same Enzymes. Journal of Experimental Medicine, v. 175, p. 567-575, 1992. | pt_BR |
| ISSN | 0022-1007 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/19476 | |
| Descrição | Pontes-de-Carvalho, L. C. “Documento produzido em parceria ou por autor vinculado à Fiocruz, mas não consta à informação no documento”. | pt_BR |
| Fomento | MacArthur Foundation, the UNDP/World Bank/WHO Special Program for Research and Training in Tropical Diseases, Conselho Nacional de Desenvolvimento Científico e Tecnologico, Secretaria de Ciencia e Tecnologia (programa RHAE), and Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (Brazil). | pt_BR |
| Idioma | eng | pt_BR |
| Editor | Rockefeller University Press | pt_BR |
| Direito Autoral | open access | pt_BR |
| Palavras-chave | Trypanosoma cruzi | pt_BR |
| Palavras-chave | Enzimas | pt_BR |
| Palavras-chave | Ácido siálico | pt_BR |
| Palavras-chave | Hidrólise | pt_BR |
| Título | Trypanosoma cruzi Trans-sialidase and Neuraminidase Activities Can Be Mediated by the Same Enzymes | pt_BR |
| Tipo do documento | Article | pt_BR |
| Resumo em Inglês | Trans-sialidase and neuraminidase activities have been detected on the surface membrane of
trypomastigotes of Trypanosoma cruzi, and both have been implicated in the parasite's invasion
of host cells. We show here that these enzymes are structurally related. They are recognized
by two independently derived monoclonal antibodies, are anchored to the membrane by
glycosylphosphatidylinositol, copurify by ion exchange, molecular sieving, and hydrophobic
chromatography, have maximal activities between pH 6.5 and 7.5, and are inactivated by heating
at 56~ Furthermore, the neuraminidase and trans-sialidase reactions are coupled. An increase
of the concentration of acceptors of the transfer reaction decreases the amount of flee sialic acid
released through the neuraminidase reaction. We conclude that a single enzyme can catalyze
the transfer or the hydrolysis of macromolecular-bound sialic acid. The predominant direction
of the reaction will depend on the availability of appropriate oligosaccharide acceptors of sialic acid. | pt_BR |
| Afiliação | Escola Paulista de Medicina. São Paulo, SP, Brasil | pt_BR |
| Afiliação | New York University Medical Center. Department of Pathology and Kaplan Cancer Center. New York, New York | pt_BR |
| Afiliação | New York University Medical Center. Department of Pathology and Kaplan Cancer Center. New York, New York | pt_BR |
| Palavras-chave em inglês | Trypanosoma cruzi | pt_BR |
| Palavras-chave em inglês | Enzymes | pt_BR |
| Palavras-chave em inglês | Sialic acid | pt_BR |
| Palavras-chave em inglês | Hydrolysis | pt_BR |
