| Author | Bezerra, Michele Alves | |
| Author | Ramos, Isabela B. | |
| Author | Paula, Iron F. de | |
| Author | Monteiro, Clarissa M. Maya | |
| Author | Klett, Eric L. | |
| Author | Coleman, Rosalind A. | |
| Author | Gondim, Katia C. | |
| Access date | 2017-07-06T11:56:02Z | |
| Available date | 2017-07-06T11:56:02Z | |
| Document date | 2017 | |
| Citation | BEZERRA, Michele Alves; et al. Deficiency of glycerol-3-phosphate acyltransferase 1 decreases triacylglycerol storage and induces fatty acid oxidation in insect fat body. Biochimica et Piophysica Acta, v.1862, p.324-336, 2017. | pt_BR |
| ISSN | 1388-1981 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/19735 | |
| Language | eng | pt_BR |
| Publisher | Elsevier | pt_BR |
| Rights | restricted access | |
| Subject in Portuguese | Lipídios | pt_BR |
| Subject in Portuguese | Beta-oxidação | pt_BR |
| Subject in Portuguese | Metabolismo dos ácidos gordurosos | pt_BR |
| Subject in Portuguese | Síntese de triacilglicerol | pt_BR |
| Subject in Portuguese | Oxidação de ácido gordo | pt_BR |
| Subject in Portuguese | Fisiologia dos Insetos | pt_BR |
| Title | Deficiency of glycerol-3-phosphate acyltransferase 1 decreases triacylglycerol storage and induces fatty acid oxidation in insect fat body | pt_BR |
| Type | Article | |
| DOI | 10.1016/j.bbalip.2016.12.004 | |
| Abstract | Glycerol-3-phosphate acyltransferases (GPAT) catalyze the initial and rate-limiting step for the de novo synthesis of triacylglycerol (TAG). Four mammalian GPAT isoforms have been identified: the mitochondria-associated GPAT1 and 2, and the endoplasmic reticulum (ER)-associated GPAT3 and 4. In the insect Rhodnius prolixus, a vector of Chagas' disease, we previously predicted a mitochondrial-like isoform (RhoprGPAT1) from genomic data. In the current study, we clone the RhoprGPAT1 coding sequence and identify an ER-associated GPAT (RhoprGPAT4) as the second isoform in the insect. RhoprGPAT1 contributes 15% of the total GPAT activity in anterior midgut, 50% in posterior midgut and fat body, and 70% in the ovary. The RhoprGpat1 gene is the predominant transcript in the midgut and fat body. To evaluate the physiological relevance of RhoprGPAT1, we generate RhoprGPAT1-deficient insects. The knockdown of RhoprGpat1 results in 50% and 65% decrease in TAG content in the posterior midgut and fat body, respectively. RhoprGpat1-deficient insects also exhibits impaired lipid droplet expansion and a 2-fold increase in fatty acid β-oxidation rates in the fat body. We propose that the RhoprGPAT1 mitochondrial-like isoform is required to channel fatty acyl chains towards TAG synthesis and away from β-oxidation. Such a process is crucial for the insect lipid homeostasis. | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica Leopoldo de Meis. Rio de Janeiro, RJ, Brasil / University of North Carolina. Department of Nutrition. Chapel Hill. USA. | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica Leopoldo de Meis. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica Leopoldo de Meis. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Affilliation | University of North Carolina. Department of Nutrition. Chapel Hill. USA / University of North Carolina. Department of Medicine. Chapel Hill, USA. | pt_BR |
| Affilliation | University of North Carolina. Department of Nutrition. Chapel Hill. USA. | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica Leopoldo de Meis. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Subject | Beta-oxidation | pt_BR |
| Subject | Fatty acid metabolism | pt_BR |
| Subject | Lipids | pt_BR |
| Subject | Triacylglycerol synthesis | pt_BR |
| Subject | Fatty acid oxidation | pt_BR |
| Subject | Insect physiology | pt_BR |
| e-ISSN | 0006-3002 | |
| Embargo date | 2030-01-01 | |
