| Author | Silveira Júnior, Lenilton Silva da | |
| Author | Silva, Franklin Souza | |
| Author | Pereira, Bernardo Acácio Santini | |
| Author | Finkelstein, Léa Cysne | |
| Author | Cavalcanti Júnior, Geraldo Barroso | |
| Author | Alves, Carlos Roberto | |
| Access date | 2017-07-06T15:29:06Z | |
| Available date | 2017-07-06T15:29:06Z | |
| Document date | 2017 | |
| Citation | SILVEIRA JUNIOR, Lenilton Silva da; et al. Exploring the Association of Surface Plasmon Resonance with Recombinant MHC:Ig Hybrid Protein as a Tool for Detecting T Lymphocytes in Mice Infected with Leishmania (Leishmania) amazonensis. BioMed Research International, Volume 2017, Article ID 9089748, 9p, 2017. | pt_BR |
| ISSN | 2314-6141 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/19743 | |
| Language | eng | pt_BR |
| Publisher | Hindawi Publishing Corporation | pt_BR |
| Rights | open access | |
| Subject in Portuguese | Leishmania | pt_BR |
| Subject in Portuguese | Doenças negligenciadas | pt_BR |
| Subject in Portuguese | Estudos de imunologia celular | pt_BR |
| Subject in Portuguese | Subconjuntos específicos de linfócitos T | pt_BR |
| Title | Exploring the Association of Surface Plasmon Resonance with Recombinant MHC:Ig Hybrid Protein as a Tool for Detecting T Lymphocytes in Mice Infected with Leishmania (Leishmania) amazonensis | pt_BR |
| Type | Article | |
| DOI | 10.1155/2017/9089748 | |
| Abstract | A surface plasmon resonance- (SPR-) based recognition method applying H-2 L(d):Ig/peptides complexes for ex vivo monitoring cellular immune responses during murine infection with Leishmania (Leishmania) amazonensis is described. Lymphocytes from lesion-draining popliteal lymph nodes were captured on a carboxylated sensor chip surface previously functionalized with H-2 L(d):Ig (DimerX) protein bound to synthetic peptides derived from the COOH-terminal region of cysteine proteinase B of L. (L.) amazonensis. In computational analysis, these peptides presented values of kinetic constants favorable to form complexes with H-2 L(d) at neutral pH, with a Gibbs free energy ΔG° < 0. The assayed DimerX:peptide complexes presented the property of attaching to distinct T lymphocytes subsets, obtained from experimentally infected BALB/c mice, in each week of infection, thus indicating a temporal variation in specific T lymphocytes populations, each directed to a different COOH-terminal region-derived peptide. The experimental design proposed herein is an innovative approach for cellular immunology studies of a neglected disease, providing a useful tool for the analysis of specific T lymphocytes subsets. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular e Doenças Endêmicas. Rio de Janeiro, RJ. Brasil / Universidade Federal do Rio Grande do Norte. Centro de Ciências da Saúde. Faculdade de Farmácia. Departamento de Análises Clínicas e Toxicológicas. Laboratório de Imunologia Clínica. Natal, RN, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular e Doenças Endêmicas. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular e Doenças Endêmicas. Rio de Janeiro, RJ. Brasil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunoparasitologia. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Affilliation | Universidade Federal do Rio Grande do Norte. Centro de Ciências da Saúde. Faculdade de Farmácia. Departamento de Análises Clínicas e Toxicológicas. Laboratório de Imunologia Clínica. Natal, RN, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular e Doenças Endêmicas. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Subject | Leishmania (Leishmania) amazonensis | pt_BR |
| Subject | neglected disease | pt_BR |
| Subject | cellular immunology studies | pt_BR |
| Subject | specific T lymphocytes subsets | pt_BR |
| xmlui.metadata.dc.subject.ods | 03 Saúde e Bem-Estar | |
