Author | Nogueira, Paula Monalisa | |
Author | Assis, Rafael Ramiro | |
Author | Torrecilhas, Ana Claudia Trocoli | |
Author | Saraiva, Elvira Maria | |
Author | Pessoa, Natália Lima | |
Author | Campos, Marco Antonio | |
Author | Santos, Eric Fabricio Marialva dos | |
Author | Ríos-Velasquez, Cláudia Maria | |
Author | Pessoa, Felipe A. | |
Author | Secundino, Nágila Francinete Costa | |
Author | Rugani, Jeronimo Marteleto Nunes | |
Author | Nieves, Elsa | |
Author | Turco, Salvatore J. | |
Author | Melo, Maria Norma | |
Author | Soares, Rodrigo Pedro Pinto | |
Access date | 2017-07-10T17:21:18Z | |
Available date | 2017-07-10T17:21:18Z | |
Document date | 2016 | |
Citation | NOGUEIRA, Paula Monalisa et al. Lipophosphoglycans from Leishmania amazonensis Strains Display Immunomodulatory Properties via TLR4 and Do Not Affect Sand Fly Infection. PLoS Neglected Tropical Diseases, v. 10, n. 8, p. 1-17, 2016. | pt_BR |
ISSN | 1935-2727 | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/19893 | |
Language | por | pt_BR |
Publisher | Public Library of Science | pt_BR |
Rights | open access | pt_BR |
Subject in Portuguese | Leishmanioses | pt_BR |
Subject in Portuguese | Leishmaniose cutanea | pt_BR |
Title | Lipophosphoglycans from Leishmania amazonensis Strains Display Immunomodulatory Properties via TLR4 and Do Not Affect Sand Fly Infection | pt_BR |
Type | Article | pt_BR |
DOI | 10.1371/journal.pntd.0004848 | |
Abstract | The immunomodulatory properties of lipophosphoglycans (LPG) from New World species of Leishmania have been assessed in Leishmania infantum and Leishmania braziliensis, the causative agents of visceral and cutaneous leishmaniasis, respectively. This glycoconjugate is highly polymorphic among species with variation in sugars that branch off the conserved Gal(β1,4)Man(α1)-PO4 backbone of repeat units. Here, the immunomodulatory activity of LPGs from Leishmania amazonensis, the causative agent of diffuse cutaneous leishmaniasis, was evaluated in two strains from Brazil. One strain (PH8) was originally isolated from the sand fly and the other (Josefa) was isolated from a human case. The ability of purified LPGs from both strains was investigated during in vitro interaction with peritoneal murine macrophages and CHO cells and in vivo infection with Lutzomyia migonei. In peritoneal murine macrophages, the LPGs from both strains activated TLR4. Both LPGs equally activate MAPKs and the NF-κB inhibitor p-IκBα, but were not able to translocate NF-κB. In vivo experiments with sand flies showed that both stains were able to sustain infection in L. migonei. A preliminary biochemical analysis indicates intraspecies variation in the LPG sugar moieties. However, they did not result in different activation profiles of the innate immune system. Also those polymorphisms did not affect infectivity to the sand fly. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, Brasil / Universidade Federal Minas Gerais. Departamento de Parasitologia. Belo Horizonte, MG, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, Brasil. | pt_BR |
Affilliation | Universidade Federal de São Paulo. Departamento de Ciências Biológicas. Laboratório de Imunologia Celular e Bioquímica de Fungos e Protozoários. São Paulo, São Paulo, Brasil. | pt_BR |
Affilliation | Universidade Federal do Rio de Janeiro. Departamento de Imunologia. Laboratório de Imunobiologia das Leishmanioses. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Leônidas e Maria Deane. Manaus, AM, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Leônidas e Maria Deane. Manaus, AM, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Leônidas e Maria Deane. Manaus, AM, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, Brazil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, Brasil. | pt_BR |
Affilliation | Universidad de Los Andes. Departamento de Biologia. Laboratório de Parasitologia Experimental. Mérida, Venezuela. | pt_BR |
Affilliation | University of Kentucky Medical Center. Department of Biochemistry. Lexington, Kentucky, USA / Liverpool School of Tropical Medicine, United Kingdom. | pt_BR |
Affilliation | Universidade Federal Minas Gerais. Departamento de Parasitologia. Belo Horizonte, MG, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, Brasil. | pt_BR |
Subject | Leishmaniasis | pt_BR |
Subject | Cutaneous Leishmaniasis | pt_BR |