| Author | Pryce, Gareth | |
| Author | dosSantos, Washington Luis Conrado | |
| Author | Male, David | |
| Access date | 2017-07-10T17:41:34Z | |
| Available date | 2017-07-10T17:41:34Z | |
| Document date | 1993 | |
| Citation | PRYCE, G.; dos SANTOS, W. L. C.; MALE, D. An assay for the analysis of lymphocyte migration across cerebral endothelium in vitro. Journal of Immunological Methods, v. 167, p. 55-63, 1993. | pt_BR |
| ISSN | 0022-1759 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/19935 | |
| Description | dos Santos, Washington Luis Carlos “Documento produzido em parceria ou por autor vinculado à Fiocruz, mas não consta à informação no documento”. | pt_BR |
| Sponsorship | Medical Research Council of Great Britain | pt_BR |
| Language | eng | pt_BR |
| Publisher | Elsevier | pt_BR |
| Rights | open access | pt_BR |
| Subject in Portuguese | Endotélio | pt_BR |
| Subject in Portuguese | Linfócitos | pt_BR |
| Subject in Portuguese | Migração | pt_BR |
| Subject in Portuguese | Adesão | pt_BR |
| Title | An assay for the analysis of lymphocyte migration across cerebral endothelium in vitro | pt_BR |
| Type | Article | pt_BR |
| Abstract | We describe a recently developed assay for the analysis of leukocyte migration across cerebral
endothelium in vitro. The endothelium is grown as monolayers on Goretex or Cyclopore membranes
coated with extracellular matrix proteins and supported on inserts. This system permits the recovery and
phenotyping of cells which migrate down through the endothelium. Using labelled lymphocytes we were
able to differentiate four populations of cells, with differing degrees of mobility in the migration assay.
We have compared the results from this system with those from conventional adhesion assays. Binding of
cells to the endothelium is rapid, but is confined to a particular subpopulation of the applied
lymphocytes. We have followed cell migration over 24 h in the system using normal and cytokine-activated
endothelium and have found that whereas adhesion depends both on the state of lymphocyte activation
and on the condition of the endothelium, the level of migration of stimulated lymphocytes is largely
independent of endothelial activation. Moreover, whereas CD8 ÷ cells bind well to the endothelium, it is
the CD4 ÷ cells which migrate most effectively. Comparison of brain and epididymal fat endothelium
showed similar migration levels over 2 h, but migration was greater across epididymal fat endothelium at
24 h. | pt_BR |
| Affilliation | Institute of Psychiatry. Department of Neuropathology. London, UK | pt_BR |
| Affilliation | Institute of Psychiatry. Department of Neuropathology. London, UK | pt_BR |
| Affilliation | Institute of Psychiatry. Department of Neuropathology. London, UK | pt_BR |
| Subject | Endothelium | pt_BR |
| Subject | Lymphocyte | pt_BR |
| Subject | Migration | pt_BR |
| Subject | Adhesion | pt_BR |
