| Author | Cavados, Clara de Fátima Gomes | |
| Author | Pires, Eder Soares | |
| Author | Chaves, Jeane Quintanilha | |
| Author | Alvarez, Danielle Nunes | |
| Author | Gil, Helio Benites | |
| Author | Oliveira, Iris Braz Ribeiro de | |
| Author | Pinto, Andrea de Barros | |
| Author | Cunha, Viviani | |
| Author | Coutinho, Carlos José Pereira da Cunha de Araújo | |
| Access date | 2017-07-11T17:21:42Z | |
| Available date | 2017-07-11T17:21:42Z | |
| Document date | 2017 | |
| Citation | CAVADOS, Clara de Fátima Gomes; et al. Isolation and genetic characterization of Lysinibacillus sphaericus strains found in mosquito larvae (Diptera: Culicidae). Research and Reports in Tropical Medicine, v.8, p.17-20, 2017. | pt_BR |
| ISSN | 1179-7282 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/20045 | |
| Language | eng | pt_BR |
| Publisher | Dove Medical Press | pt_BR |
| Rights | open access | |
| Subject in Portuguese | Culicidae | pt_BR |
| Subject in Portuguese | Mosquitos | pt_BR |
| Subject in Portuguese | Bioensaios qualitativos | pt_BR |
| Subject in Portuguese | Bactéria entomopatogênica | pt_BR |
| Subject in Portuguese | Reação em Cadeia da Polimerase em Tempo Real | pt_BR |
| Subject in Portuguese | Lysinibacillus sphaericus | pt_BR |
| Title | Isolation and genetic characterization of Lysinibacillus sphaericus strains found in mosquito larvae (Diptera: Culicidae) | pt_BR |
| Type | Article | |
| DOI | 10.2147/RRTM.S124066 | |
| Abstract | Introduction: Lysinibacillus sphaericus is a highly effective and specific bioinsecticide used
for the control of Culicidae larvae.
Objective: This study aimed to identify and characterize L. sphaericus strains isolated from
Culex quinquefasciatus larvae in Brazil.
Methods: C. quinquefasciatus larvae were collected from streams in the urban area of São Paulo
state. L. sphaericus strains were identified through cytomorphology, biochemical, and physiological
analyses. Qualitative bioassays were performed to evaluate the toxicity of the strains against C.
quinquefasciatus. The crystal compound protein pattern of L. sphaericus strains was analyzed by
sodium dodecyl sulfate polyacrylamide gel electrophoresis. Five reference strains were used as
standards in all tests performed. Repetitive extragenic palindromic elements-polymerase chain reaction
(REP-PCR) was utilized in an attempt to differentiate pathogenic and nonpathogenic isolates.
Results: Twenty-one strains were isolated. Only one presented toxic activity against C. quinquefasciatus.
REP-PCR results identified 23 patterns among the 26 strains used in the study,
and the fragment analysis showed low similarity (16%) between L. sphaericus isolates and the
five reference strains.
Conclusion: Comparison of strains isolated in this study using REP-PCR showed a low similarity
to other strains, demonstrating the high intraspecific variability for L. sphaericus. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Fisiologia Bacteriana. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Fisiologia Bacteriana. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Fisiologia Bacteriana. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Fisiologia Bacteriana. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Affilliation | Superintendência de Controle de Endemias. São Paulo, SP, Brasil. | pt_BR |
| Affilliation | Superintendência de Controle de Endemias. São Paulo, SP, Brasil. | pt_BR |
| Affilliation | Superintendência de Controle de Endemias. São Paulo, SP, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Fisiologia Bacteriana. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Subject | Lysinibacillus sphaericus | pt_BR |
| Subject | Culicidae | pt_BR |
| Subject | qualitative bioassays | pt_BR |
| Subject | REP-PCR | pt_BR |
| Subject | mosquitoes | pt_BR |
| Subject | entomopathogenic bacteria | pt_BR |
