| Author | Cosate, Maria Raquel Venturim | |
| Author | Sakamoto, Tetsu | |
| Author | Mendes, Tiago Antônio de Oliveira | |
| Author | Moreira, Élvio Carlos | |
| Author | Silva, Carlos Gustavo Regis da | |
| Author | Brasil, Bruno dos Santos Alves Figueiredo | |
| Author | Oliveira, Camila Stefanie Fonseca de | |
| Author | Azevedo, Vasco Ariston de Carvalho | |
| Author | Ortega, José Miguel | |
| Author | Leite, Rômulo Cerqueira | |
| Author | Haddad, João Paulo | |
| Access date | 2017-07-13T19:18:37Z | |
| Available date | 2017-07-13T19:18:37Z | |
| Document date | 2017 | |
| Citation | COSATE, M. R. V. et al. Molecular typing of Leptospira interrogans serovar Hardjo isolates from leptospirosis outbreaks in Brazilian livestock. BMC Veterinary Research, v. 13, p. 177, 2017. | pt_BR |
| ISSN | 1746-6148 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/20085 | |
| Sponsorship | Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) and Instituto Nacional de Ciência e Tecnologia (INCT) de Informação Genético-Sanitária da Pecuária Brasileira | pt_BR |
| Language | eng | pt_BR |
| Publisher | BioMed Central | pt_BR |
| Rights | open access | pt_BR |
| Subject in Portuguese | Leptospira interrogans | pt_BR |
| Subject in Portuguese | Leptospirose | pt_BR |
| Subject in Portuguese | Gado | pt_BR |
| Subject in Portuguese | Animais | pt_BR |
| Title | Molecular typing of Leptospira interrogans serovar Hardjo isolates from leptospirosis outbreaks in Brazilian livestock | pt_BR |
| Type | Article | pt_BR |
| DOI | 10.1186/s12917-017-1081-9 | |
| Abstract | Leptospirosis is caused by pathogenic spirochetes of the genus Leptospira spp. This zoonotic disease is distributed globally and affects domestic animals, including cattle. Leptospira interrogans serogroup Sejroe serovar Hardjo and Leptospira borgpetersenii serogroup Sejroe serovar Hardjo remain important species associated with this reproductive disease in livestock production. Previous studies on Brazilian livestock have reported that L. interrogans serovar Hardjo is the most prevalent leptospiral agent in this country and is related to clinical signs of leptospirosis, which lead to economic losses in production. Here, we described the isolation of three clinical strains (Norma, Lagoa and Bolivia) obtained from leptospirosis outbreaks that occurred in Minas Gerais state in 1994 and 2008. Results: Serological and molecular typing using housekeeping (secY and 16SrRNA) and rfb locus (ORF22 and ORF36)
genes were applied for the identification and comparative analysis of Leptospira spp. Our results identified the three
isolates as L. interrogans serogroup Sejroe serovar Hardjo and confirmed the occurrence of this bacterial strain in
Brazilian livestock. Genetic analysis using ORF22 and ORF36 grouped the Leptospira into serogroup Sejroe and subtype
Hardjoprajitno. Genetic approaches were also applied to compare distinct serovars of L. interrogans strains by verifying
the copy numbers of the IS1500 and IS1533 insertion sequences (ISs). The IS1500 copy number varied among the
analyzed L. interrogans strains.
Conclusion: This study provides evidence that L. interrogans serogroup Sejroe serovar Hardjo subtype Hardjoprajitno
causes bovine leptospirosis in Brazilian production. The molecular results suggested that rfb locus (ORF22 and
ORF36) could improve epidemiological studies by allowing the identification of Leptospira spp. at the serogroup
level. Additionally, the IS1500 and IS1533 IS copy number analysis suggested distinct genomic features among
closely related leptospiral strains. | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, Brasil | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, Brasil | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, Brasil | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Escola de Veterinária. Departamento de Medicina Veterinária Preventiva. Belo Horizonte, MG, Brasil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Laboratório Biologia Parasitária. Salvador, BA, Brasil | pt_BR |
| Affilliation | Embrapa Agroenergia. Brasília, DF, Brasil | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Escola de Veterinária. Departamento de Medicina Veterinária Preventiva. Belo Horizonte, MG, Brasil | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas de Minas Gerais. Departamento de Genética. Belo Horizonte, MG, Brasil | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, Brasil | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Escola de Veterinária. Departamento de Medicina Veterinária Preventiva. Belo Horizonte, MG, Brasil | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Escola de Veterinária. Departamento de Medicina Veterinária Preventiva. Belo Horizonte, MG, Brasil | pt_BR |
| Subject | Leptospira interrogans | pt_BR |
| Subject | Leptospirosis | pt_BR |
| Subject | Livestock | pt_BR |
| Subject | Animals | pt_BR |
