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EVALUATION OF THE ANTI-LIPL32 MONOCLONAL ANTIBODIES POTENTIAL FOR USE IN LEPTOSPIROSIS IMMUNODIAGNOSTIC TESTS
Antígenos
Afinidade de anticorpos
Mapeamento de epítopos
ELISA
Western Blot
Recombinant antigens,
Antibody affinity
Epitope mapping
ELISA
Western blotting
Autor
Afiliación
Federal University of Pelotas. Center for Biotechnology. Porto Alegre, RGS, Brazil.
Federal University of Pelotas. Center for Biotechnology. Porto Alegre, RS, Brazil
Federal University of Pelotas. Center for Biotechnology. Porto Alegre, RS, Brazil
Federal University of Pelotas. Center for Biotechnology. Porto Alegre, RS, Brazil
Federal University of Pelotas. Center for Biotechnology. Porto Alegre, RS, Brazil
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil.
Federal University of Pelotas. Center for Biotechnology. Porto Alegre, RS, Brazil.
Federal University of Pelotas. Center for Biotechnology. Porto Alegre, RS, Brazil.
Federal University of Pelotas. Center for Biotechnology. Porto Alegre, RS, Brazil
Federal University of Pelotas. Center for Biotechnology. Porto Alegre, RS, Brazil
Federal University of Pelotas. Center for Biotechnology. Porto Alegre, RS, Brazil
Federal University of Pelotas. Center for Biotechnology. Porto Alegre, RS, Brazil
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil.
Federal University of Pelotas. Center for Biotechnology. Porto Alegre, RS, Brazil.
Federal University of Pelotas. Center for Biotechnology. Porto Alegre, RS, Brazil.
Resumen en ingles
LipL32 is the major lipoprotein in the membrane of pathogenic leptospira. In this work, we report on the production of monoclonal antibodies (MAbs) against recombinant LipL32 (rLipL32) and on the evaluation of their potential for use as reagents in diagnostic tests for leptospirosis. The MAbs were all of the IgG(2b) isotype and reacted specifically with native LipL32 in pathogenic serovars only. MAbs reacted in the same region of the rLipL32 molecule and their affinity constant was between 5x10(7) M(-1) and 6x10(6) M(-1). These results suggest that although the MAbs cannot be used together, they are well suited for diagnostic tests of leptospirosis based on LipL32 detection.
Palabras clave en portugues
LeptospiraAntígenos
Afinidade de anticorpos
Mapeamento de epítopos
ELISA
Western Blot
Palabras clave en ingles
LeptospiraRecombinant antigens,
Antibody affinity
Epitope mapping
ELISA
Western blotting
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