| Author | Santos, Danielle Silva dos | |
| Author | Albuquerque, Juliana Barreto de | |
| Author | Guerra, Bárbara | |
| Author | Moreira, Otacilio C. | |
| Author | Berbert, Luiz Ricardo | |
| Author | Ramos, Mariana Tavares | |
| Author | Mascarenhas, Barbara Angelica S. | |
| Author | Britto, Constança | |
| Author | Morrot, Alexandre | |
| Author | Villa-Verde, Déa M. Serra | |
| Author | Garzoni, Luciana Ribeiro | |
| Author | Savino, Wilson | |
| Author | Almeida, Vinicius Cotta de | |
| Author | Meis, Juliana de | |
| Access date | 2017-07-20T11:38:43Z | |
| Available date | 2017-07-20T11:38:43Z | |
| Document date | 2017 | |
| Citation | SANTOS, Danielle Silva dos; et al. Unraveling Chagas disease transmission through the oral route: Gateways to Trypanosoma cruzi infection and target tissues. PLoS Negl Trop Dis., v.11, n.4, e0005507, 26p, Apr. 2017. | pt_BR |
| ISSN | 1935-2727 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/20153 | |
| Language | eng | pt_BR |
| Publisher | Public Library of Science | pt_BR |
| Rights | open access | |
| Subject in Portuguese | Trypanosoma cruzi | pt_BR |
| Subject in Portuguese | Doença de Chagas | pt_BR |
| Subject in Portuguese | Transmissão oral | pt_BR |
| Title | Unraveling Chagas disease transmission through the oral route: Gateways to Trypanosoma cruzi infection and target tissues | pt_BR |
| Type | Article | |
| DOI | 10.1371/journal. pntd.0005507 | |
| Abstract | Oral transmission of Trypanosoma cruzi, the causative agent of Chagas disease, is the
most important route of infection in Brazilian Amazon and Venezuela. Other South American
countries have also reported outbreaks associated with food consumption. A recent study
showed the importance of parasite contact with oral cavity to induce a highly severe acute
disease in mice. However, it remains uncertain the primary site of parasite entry and multiplication
due to an oral infection. Here, we evaluated the presence of T. cruzi Dm28c luciferase
(Dm28c-luc) parasites in orally infected mice, by bioluminescence and quantitative real-time
PCR. In vivo bioluminescent images indicated the nasomaxillary region as the site of parasite
invasion in the host, becoming consistently infected throughout the acute phase. At later
moments, 7 and 21 days post-infection (dpi), luminescent signal is denser in the thorax,
abdomen and genital region, because of parasite dissemination in different tissues. Ex vivo
analysis demonstrated that the nasomaxillary region, heart, mandibular lymph nodes, liver,
spleen, brain, epididymal fat associated to male sex organs, salivary glands, cheek muscle,
mesenteric fat and lymph nodes, stomach, esophagus, small and large intestine are target
tissues at latter moments of infection. In the same line, amastigote nests of Dm28c GFP T.
cruzi were detected in the nasal cavity of 6 dpi mice. Parasite quantification by real-time
qPCR at 7 and 21 dpi showed predominant T. cruzi detection and expansion in mouse nasal
cavity. Moreover, T. cruzi DNA was also observed in the mandibular lymph nodes, pituitary
gland, heart, liver, small intestine and spleen at 7 dpi, and further, disseminated to other tissues,
such as the brain, stomach, esophagus and large intestine at 21 dpi. Our results
clearly demonstrated that oral cavity and adjacent compartments is the main target region in
oral T. cruzi infection leading to parasite multiplication at the nasal cavity. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa sobre o Timo. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa sobre o Timo. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Centro Nacional de Biologia Estrutural e Bioimagem. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular e Doenças Endêmicas. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa sobre o Timo. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa sobre o Timo. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa sobre o Timo. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular e Doenças Endêmicas. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes. Departamento de Imunologia. Rio de Janeiro, RJ, Brasil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa sobre o Timo. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Inovações em Terapias, Ensino e Bioprodutos. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa sobre o Timo. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa sobre o Timo. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa sobre o Timo. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Subject | Trypanosoma cruzi | pt_BR |
| Subject | Chagas disease | pt_BR |
| Subject | Oral transmission | pt_BR |
| e-ISSN | 1935-2735 | |
| xmlui.metadata.dc.subject.ods | 03 Saúde e Bem-Estar | |
