| Author | Alves, Carlos Roberto | |
| Author | Pontes-de-Carvalho, Lain Carlos | |
| Author | Souza, André Luis Almeida | |
| Author | De Simone, Salvatore Giovanni | |
| Access date | 2017-07-25T13:16:34Z | |
| Available date | 2017-07-25T13:16:34Z | |
| Document date | 2001 | |
| Citation | ALVES, Carlos Roberto et al. A strategy for the identification of T-cell epitopes on Leishmania cysteine proteinases. Cytobios, v. 104, p. 33-41, 2001. | pt_BR |
| ISSN | 0011-4529 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/20388 | |
| Sponsorship | Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), Fundação de Amparo à Pesquisa do estado do Rio de Janeiro (FAPERJ) and FIOCRUZ. | pt_BR |
| Language | eng | pt_BR |
| Rights | open access | |
| Subject in Portuguese | Leishmania | pt_BR |
| Subject in Portuguese | Cisteina proteinases | pt_BR |
| Subject in Portuguese | Epitopos | pt_BR |
| Subject in Portuguese | Leishmania braziliensis | pt_BR |
| Subject in Portuguese | Humanos | pt_BR |
| Title | A strategy for the identification of T-cell epitopes on Leishmania cysteine proteinases | pt_BR |
| Type | Article | |
| Abstract | In this study computational analysis was used to compile sequence alignments, constructo a dendrogram and calculate physical data in order to predict potential T-cell epitopes of the Leishmania cysteine proteinase. Using multiple alignment of human and Leishmania proteinase sequences deposited on data bank sequences, it was possible to predict that the extreme C-terminus of cysteine proteinase (Cyspep, 355-444) contained three peptides (pl 361-370, pll 415-422 and plll 431-444) with charge score, hydrophobicity and isoelectric points compatible for human leucocyte-associated antigen (HLA) class II binding. The prediction was confirmed in vitro through the ability of synthetic peptides corresponding to the predicted regions to stimulate peripheral blood mononuclear cells of patients with leishmaniasis. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Bioquímica de Proteinases e Peptídeos. Departamento de Bioquímica e Biologia Molecular. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Laboratório de Imunologia Molecular. Salvador, BA, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Bioquímica de Proteinases e Peptídeos. Departamento de Bioquímica e Biologia Molecular. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Bioquímica de Proteinases e Peptídeos. Departamento de Bioquímica e Biologia Molecular. Rio de Janeiro, RJ, Brasil / Universidade Federal Fluminense. Instituto de Biologia. Departamento de Biologia Celular e Molecular. Niterói, RJ, Brasil. | pt_BR |
| Subject | Leishmania | pt_BR |
| Subject | Cysteine proteinases | pt_BR |
| Subject | Epitopes | pt_BR |
| Subject | Leishmania braziliensis | pt_BR |
| Subject | Humans | pt_BR |
