| Author | Drehmer, Daiana Leila | |
| Author | Aguiar, Alessandra Melo de | |
| Author | Brandt, Anna Paula | |
| Author | Petiz, Lyvia | |
| Author | Cadena, Sílvia Maria Suter Correia | |
| Author | Rebelatto, Carmem K. | |
| Author | Brofman, Paulo Roberto Slud | |
| Author | Filipak Neto, Francisco | |
| Author | Dallagiovanna, Bruno | |
| Author | Abud, Ana Paula Ressetti | |
| Access date | 2017-07-25T18:48:05Z | |
| Available date | 2017-07-25T18:48:05Z | |
| Document date | 2016 | |
| Citation | DREHMER, Daiana Leila et al. Metabolic switches during the first steps of adipogenic stem cells differentiation. Stem Cell Research. v. 17, n. 2, p. 413-421, Sept. 2016. | pt_BR |
| ISSN | 1876-7753 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/20405 | |
| Language | por | pt_BR |
| Publisher | Elsevier | pt_BR |
| Rights | open access | pt_BR |
| Subject in Portuguese | Citoproteção | pt_BR |
| Title | Metabolic switches during the first steps of adipogenic Stem Cells differentiation | pt_BR |
| Type | Article | pt_BR |
| DOI | 10.1016/j.scr.2016.09.001 | pt_BR |
| Abstract | The understanding of metabolism during cell proliferation and commitment provides a greater insight into the basic biology of cells, allowing future applications. Here we evaluated the energy and oxidative changes during
the early adipogenic differentiation of human adipose tissue-derived stromal cells (hASCs). hASCs were maintained under differentiation conditions during 3 and 7 days. Oxygen consumption, mitochondrial mass and
membrane potential, reactive oxygen species (ROS) generation, superoxide dismutase (SOD) and catalase activities, non-protein thiols (NPT) concentration and lipid peroxidation were analyzed. We observed that 7 days of adipogenic induction are required to stimulate cells to consume more oxygen and increase mitochondrial activity, indicating organelle maturation and a transition from glycolytic to oxidative energy metabolism. ROS production was only increased after 3 days and may be involved in the differentiation commitment. ROS source was not only the mitochondria and we suggest that NOX proteins are related to ROS generation and therefore adipogenic commitment. ROS production did not change after 7 days, but an increased activity of catalase and NPT concentration as well as a decreased lipid peroxidation were observed. Thus, a short period of differentiation induction is able to change the energetic and oxidative metabolic profile of hASCs and stimulate cytoprotection processes. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Básica de Células Tronco. Curitiba, PR, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Básica de Células Tronco. Curitiba, PR, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Paraná. Paraná, PR, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Paraná. Paraná, PR, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Paraná. Paraná, PR, Brasil. | pt_BR |
| Affilliation | Pontifícia Universidade Católica do Paraná. Curitiba, PR, Brasil. | pt_BR |
| Affilliation | Pontifícia Universidade Católica do Paraná. Curitiba, PR, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Paraná. Paraná, PR, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Básica de Células Tronco. Curitiba, PR, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Básica de Células Tronco. Curitiba, PR, Brasil. | pt_BR |
| Subject | Adult Stem Cells | pt_BR |
| Subject | Adipogenesis | pt_BR |
| Subject | Metabolism | pt_BR |
| Subject | Reactive Oxygen Species | pt_BR |
| Subject | Antioxidants | pt_BR |
| Subject | Cytoprotection | pt_BR |
| Subject in Spanish | Células Madre Adultas | pt_BR |
| Subject in Spanish | Adipogénesis | pt_BR |
| Subject in Spanish | Metabolismo | pt_BR |
| Subject in Spanish | Especies Reactivas de Oxígeno | pt_BR |
| Subject in Spanish | Antioxidantes | pt_BR |
| Subject in Spanish | Citoprotección | pt_BR |
| DeCS | Células-Tronco Adultas | pt_BR |
| DeCS | Adipogenia | pt_BR |
| DeCS | Metabolismo | pt_BR |
| DeCS | Espécies Reativas de Oxigênio | pt_BR |
| DeCS | Antioxidantes | pt_BR |
