| Author | Moreira, Marcela de Lima | |
| Author | Pereira, Christiane Costa | |
| Author | Alves, Marina Luiza Rodrigues | |
| Author | Marteleto, Bruno Henrique | |
| Author | Ribeiro, Vitor Marcio | |
| Author | Pascoal, Vanessa Peruhype Magalhães | |
| Author | Giunchetti, Rodolfo Cordeiro | |
| Author | Martins Filho, Olindo Assis | |
| Author | Araújo, Márcio Sobrera Silva | |
| Access date | 2017-07-27T17:21:57Z | |
| Available date | 2017-07-27T17:21:57Z | |
| Document date | 2016 | |
| Citation | MOREIRA, Marcela de Lima et al. Vaccination against canine leishmaniosis increases the phagocytic activity, nitric oxide production and expression of cell activation/migration molecules in neutrophils and monocytes. Vet Parasitol. v. 220, p. 33-45, 2016 | pt_BR |
| ISSN | 0304-4017 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/20426 | |
| Language | eng | pt_BR |
| Publisher | Elsevier | pt_BR |
| Rights | restricted access | pt_BR |
| Subject in Portuguese | Leishmanioses | pt_BR |
| Subject in Portuguese | vacina | pt_BR |
| Title | Vaccination against canine leishmaniosis increases the phagocytic activity, nitric oxide production and expression of cell activation/migration molecules in neutrophils and monocytes | pt_BR |
| Type | Article | |
| DOI | 10.1016/j.vetpar.2016.02.009 | |
| Abstract | Visceral leishmaniasis (VL) is transmitted by phlebotomine sandfly vectors and domestic dogs serve as a reservoir. The elimination of seropositive dogs has been a recommended strategy for managing the disease in Brazil. A protective canine vaccine would be an important tool for controlling the disease, reducing the parasites available to sandfly vectors and, consequently, reducing the number of human VL cases. Leishmune® is an anti-canine Leishmaniosis (VL Canine) vaccine produced by Zoetis (Pfizer, Brazil) that was commercially available in Brazil until 2014. The main goal of the present study was to investigate the protective immunological events induced by vaccination with Leishmune® in the time frame of one year. Healthy, non-vaccinated dogs and dogs of 1, 6 and 10 months post-vaccination were evaluated. Results showed that Leishmune® induced an increase in phagocytic activity of neutrophils and monocytes and also increased NO production. Immunological events were correlated with functional responses, as high levels of IgG and an increase of the receptor Fcγ were detected. Vaccination induced an increased expression of TLR (2, 4, 5, 9), integrin (CD29, CD49f), activation (MHCII) and co-stimulatory (CD80, CD81) molecules by neutrophils and monocytes. Vaccination led to decrease of IL-4 and an increase of IL-8 production by monocytes and higher IFN-γ and IL-17 production by T-cells. The results suggested that Leishmune® was able to induce a long-lasting change in immune response, mediated by supportive immunological events that may be participating in protective immunity against CL. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Biomarcadores de Diagnóstico e Monitoração. Belo Horizonte, MG, Brazil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Biomarcadores de Diagnóstico e Monitoração. Belo Horizonte, MG, Brazil | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Departamento de Morfologia. Laboratório de Biologia das Interações Celulares. Belo Horizonte, MG, Brazil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Biomarcadores de Diagnóstico e Monitoração. Belo Horizonte, MG, Brazil | pt_BR |
| Affilliation | Clínica Veterinária Santo Agostinho. Belo Horizonte, MG, Brazil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Biomarcadores de Diagnóstico e Monitoração. Belo Horizonte, MG, Brazil | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Departamento de Morfologia. Laboratório de Biologia das Interações Celulares. Belo Horizonte, MG, Brazil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Esquistossomose. Belo Horizonte, MG, Brasil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Biomarcadores de Diagnóstico e Monitoração. Belo Horizonte, MG, Brazil | pt_BR |
| Subject | Vaccine | pt_BR |
| Subject | Leishmune® | pt_BR |
| Subject | Canine leishmaniosis | pt_BR |
| Subject | Leishmania infantum | pt_BR |
| Subject | Phagocytosis | pt_BR |
| Subject | TLRs | pt_BR |
| Subject | Cytokines | pt_BR |
| Embargo date | 2025-01-01 | |
| xmlui.metadata.dc.subject.ods | 03 Saúde e Bem-Estar | |
