Author | Lima, D. C. | |
Author | Duarte, Fábio T. | |
Author | Medeiros, Viviane Katielly Silva | |
Author | Carvalho, Paulo Costa | |
Author | Nogueira, Fábio Cesar Sousa | |
Author | Araujo, Gabriel Duarte Tavares | |
Author | Dumont, Gilberto Barbosa | |
Author | Medeiros, Sivia Regina Batistuzzo de | |
Access date | 2017-07-28T16:16:46Z | |
Available date | 2017-07-28T16:16:46Z | |
Document date | 2016 | |
Citation | LIMA, D. C. et al. GeLC-MS-based proteomics of Chromobacterium violaceum: comparison of proteome changes elicited by hydrogen peroxide. Scientific Reports, v. 6, n. 28174, p. 1-12, Jun. 16. | pt_BR |
ISSN | 2045-2322 | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/20446 | |
Language | eng | pt_BR |
Publisher | Nature Publishing Group | pt_BR |
Rights | open access | pt_BR |
Subject in Portuguese | Proteômica | pt_BR |
Title | GeLC-MS-based proteomics of Chromobacterium violaceum: comparison of proteome changes elicited by hydrogen peroxide | pt_BR |
Type | Article | pt_BR |
DOI | 10.1038/srep28174 | pt_BR |
Abstract | Chromobacterium violaceum is a free-living bacillus with several genes that enables it survival under
different harsh environments such as oxidative and temperature stresses. Here we performed a labelfree
quantitative proteomic study to unravel the molecular mechanisms that enable C. violaceum to
survive oxidative stress. To achieve this, total proteins extracted from control and C. violaceum cultures
exposed during two hours with 8mM hydrogen peroxide were analyzed using GeLC-MS proteomics.
Analysis revealed that under the stress condition, the bacterium expressed proteins that protected
it from the damage caused by reactive oxygen condition and decreasing the abundance of proteins
responsible for bacterial growth and catabolism. GeLC-MS proteomics analysis provided an overview
of the metabolic pathways involved in the response of C. violaceum to oxidative stress ultimately
aggregating knowledge of the response of this organism to environmental stress. This study identified
approximately 1500 proteins, generating the largest proteomic coverage of C. violaceum so far. We also
detected proteins with unknown function that we hypothesize to be part of new mechanisms related to
oxidative stress defense. Finally, we identified the mechanism of clustered regularly interspaced short
palindromic repeats (CRISPR), which has not yet been reported for this organism. | pt_BR |
Affilliation | Universidade Federal do Rio Grande do Norte. Centro de Biociências. Laboratório de Biologia Molecular e Genômica. Natal, RN, Brasil. | pt_BR |
Affilliation | Instituto Federal de Educação, Ciência e Tecnologia do Rio Grande do Norte (IFRN). RN, Brasil. | pt_BR |
Affilliation | Universidade Federal do Vale do São Francisco. Colegiado de Medicina. Petrolina, PE, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Proteômica e Engenharia de Proteínas. Curitiba, PR, Brasil. | pt_BR |
Affilliation | Universidade Federal do Rio de Janeiro. Centro de Tecnologia. Laboratório de Química de Proteínas. Unidade Proteômica. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Universidade Federal do Rio de Janeiro. Centro de Tecnologia. Laboratório de Química de Proteínas. Unidade Proteômica. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Universidade Federal do Rio de Janeiro. Centro de Tecnologia. Laboratório de Química de Proteínas. Unidade Proteômica. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Universidade Federal do Rio Grande do Norte. Centro de Biociências. Laboratório de Biologia Molecular e Genômica. Natal, RN, Brasil. | pt_BR |
Subject | Chromobacterium | pt_BR |
Subject | Proteomics | pt_BR |
Subject | Clustered Regularly Interspaced Short Palindromic Repeats | pt_BR |
Subject | Hydrogen Peroxide | pt_BR |
Subject in Spanish | Chromobacterium | pt_BR |
Subject in Spanish | Proteómica | pt_BR |
Subject in Spanish | Repeticiones Palindrómicas Cortas Agrupadas y Regularmente Espaciadas | pt_BR |
Subject in Spanish | Peróxido de Hidrógeno | pt_BR |
DeCS | Chromobacterium | pt_BR |
DeCS | Repetições Palindrômicas Curtas Agrupadas e Regularmente Espaçadas | pt_BR |
DeCS | Peróxido de Hidrogênio | pt_BR |