Author | Passaes, Caroline Pereira Bittencourt | |
Author | Bello, Gonzalo | |
Author | Lorete, Roberta S. | |
Author | Almeida, Sabrina E. Matos | |
Author | Junqueira, Dennis Maletich | |
Author | Morgado, Mariza G. | |
Access date | 2017-08-15T13:51:35Z | |
Available date | 2017-08-15T13:51:35Z | |
Document date | 2009 | |
Citation | PASSAES, Carolina Pereira Bittencourt; et al. Genetic characterization of HIV-1 BC recombinants and evolutionary history of the CRF31_BC in Southern Brazil. Infection, Genetics and Evolution, v.9, p.474-482, 9p, 2009. | pt_BR |
ISSN | 1567-1348 | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/20614 | |
Language | eng | pt_BR |
Publisher | Elsevier | pt_BR |
Rights | restricted access | pt_BR |
Subject in Portuguese | HIV-1 | pt_BR |
Subject in Portuguese | Brasil | pt_BR |
Subject in Portuguese | História da evolução | pt_BR |
Subject in Portuguese | Subtipo C | pt_BR |
Subject in Portuguese | Recombinantes de segunda geração | pt_BR |
Title | Genetic characterization of HIV-1 BC recombinants and evolutionary history of the CRF31_BC in Southern Brazil | pt_BR |
Type | Article | pt_BR |
DOI | 10.1016/j.meegid.2009.01.008 | |
Abstract | To evaluate the recombination profiles and evolutionary history of HIV-1 BC recombinants in Southern Brazil, 81 isolates collected in the city of Porto Alegre (Rio Grande do Sul State) from 1998 to 2006 previously subtyped as C (env-gp120/C2V3) were screened in the protease-reverse transcriptase (pr/rt), integrase and gp41 genomic regions. Detailed phylogenetic, bootscan and informative site analyses were performed to trace the subtype classification. The evolutionary rate and divergence time of the Brazilian CRF31_BC epidemic were estimated using a Bayesian Markov Chain Monte Carlo framework. Analysis of the four target regions identified: 43 isolates as "pure" subtype C, 23 as CRF31_BC, and 15 as unique BC recombinant forms (URFs_BC). Recombination breakpoints were mainly localized in the rt gene and 100% of the recombinant samples could be detected analyzing only this region. Most URFs_BC (86.7%) contained small subtype B fragments (<or=160nt) in the rt region and shared one of the recombination breakpoints with CRF31_BC. In conclusion, despite the high co-prevalence of subtypes B and C in Porto Alegre, the diversity of BC recombinant forms circulating in this region was extremely low. Most BC recombinants were CRF31_BC and URFs_BC that appeared to be second generation recombinants derived from CRF31_BC and subtype C strains, confirming the importance of this CRF in this region. The emergence of the CRF31_BC was estimated to be around 1988 (1982-1992). | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de AIDS e Imunologia Molecular. Rio de Janeiro, RJ. Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de AIDS e Imunologia Molecular. Rio de Janeiro, RJ. Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de AIDS e Imunologia Molecular. Rio de Janeiro, RJ. Brasil. | pt_BR |
Affilliation | Fundação Estadual de Produção e Pesquisa em Saúde. Centro de Desenvolvimento Científico e Tenológico. Porto Alegre, RS, Brasil. | pt_BR |
Affilliation | Fundação Estadual de Produção e Pesquisa em Saúde. Centro de Desenvolvimento Científico e Tecnológico. Porto Alegre, RS, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Rio de Janeiro, RJ. Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de AIDS e Imunologia Molecular. Rio de Janeiro, RJ. Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de AIDS e Imunologia Molecular. Rio de Janeiro, RJ. Brasil. | pt_BR |
Subject | Brazil | pt_BR |
Subject | HIV-1 | pt_BR |
Subject | Subtype C | pt_BR |
Subject | CRF51_BC | pt_BR |
Subject | Second generation recombinants | pt_BR |
Subject | Evolutionary history | pt_BR |
Embargo date | 2030-01-01 | |