| Author | Souza, Bruno Solano de Freitas | |
| Author | Silva, Kátia Nunes da | |
| Author | Silva, Daniela Nascimento | |
| Author | Rocha, Vinícius Pinto Costa | |
| Author | Paredes, Bruno Diaz | |
| Author | Azevedo, Carine Machado | |
| Author | Nonaka, Carolina Kymie | |
| Author | Carvalho, Gisele Batista | |
| Author | Vasconcelos, Juliana Fraga | |
| Author | Santos, Ricardo Ribeiro dos | |
| Author | Soares, Milena Botelho Pereira | |
| Access date | 2017-08-15T18:48:18Z | |
| Available date | 2017-08-15T18:48:18Z | |
| Document date | 2017 | |
| Citation | SOUZA, B. S. F. et al. Galectin-3 Knockdown Impairs Survival, Migration, and Immunomodulatory Actions of Mesenchymal Stromal Cells in a Mouse Model of Chagas Disease Cardiomyopathy. Stem Cells International, 3282656, 2017. | pt_BR |
| ISSN | 1687-966X | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/20636 | |
| Sponsorship | CNPq, FAPESB, and FINEP. | pt_BR |
| Language | eng | pt_BR |
| Publisher | Hindawi Publishing Corporation | pt_BR |
| Rights | open access | pt_BR |
| Subject in Portuguese | Doença de Chagas | pt_BR |
| Subject in Portuguese | Cardiomiopatia chagasica | pt_BR |
| Subject in Portuguese | Transplante de celulas | pt_BR |
| Subject in Portuguese | Trypanosoma cruzi | pt_BR |
| Subject in Portuguese | Animais | pt_BR |
| Subject in Portuguese | Ratos | pt_BR |
| Title | Galectin-3 Knockdown Impairs Survival, Migration, and Immunomodulatory Actions of Mesenchymal Stromal Cells in a Mouse Model of Chagas Disease Cardiomyopathy | pt_BR |
| Type | Article | pt_BR |
| DOI | 10.1155/2017/3282656 | |
| Abstract | Therapies based on transplantation of mesenchymal stromal cells (MSC) hold promise for the management of inflammatory disorders. In chronic Chagas disease cardiomyopathy (CCC), caused by chronic infection with Trypanosoma cruzi, the exacerbated immune response plays a critical pathophysiological role and can be modulated by MSC. Here, we investigated the role of galectin-3 (Gal-3), a beta-galactoside-binding lectin with several actions on immune responses and repair process, on the immunomodulatory potential of MSC. Gal-3 knockdown in MSC did not affect the immunophenotype or differentiation potential. However, Gal-3 knockdown MSC showed decreased proliferation, survival, and migration. Additionally, when injected intraperitoneally into mice with CCC, Gal-3 knockdown MSC showed impaired migration in vivo. Transplantation of control MSC into mice with CCC caused a suppression of cardiac inflammation and fibrosis, reducing expression levels of CD45, TNFα, IL-1β, IL-6, IFNγ, and type I collagen. In contrast, Gal-3 knockdown MSC were unable to suppress the immune response or collagen synthesis in the hearts of mice with CCC. Finally, infection with T. cruzi demonstrated parasite survival in wild-type but not in Gal-3 knockdown MSC. These findings demonstrate that Gal-3 plays a critical role in MSC survival, proliferation, migration, and therapeutic potential in CCC. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / São Rafael Hospital. Center for Biotechnology and Cell Therapy. Salvador, BA, Brazil | pt_BR |
| Affilliation | São Rafael Hospital. Center for Biotechnology and Cell Therapy. Salvador, BA, Brazil | pt_BR |
| Affilliation | São Rafael Hospital. Center for Biotechnology and Cell Therapy. Salvador, BA, Brazil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
| Affilliation | São Rafael Hospital. Center for Biotechnology and Cell Therapy. Salvador, BA, Brazil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / São Rafael Hospital. Center for Biotechnology and Cell Therapy. Salvador, BA, Brazil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / São Rafael Hospital. Center for Biotechnology and Cell Therapy. Salvador, BA, Brazil | pt_BR |
| Affilliation | São Rafael Hospital. Center for Biotechnology and Cell Therapy. Salvador, BA, Brazil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / São Rafael Hospital. Center for Biotechnology and Cell Therapy. Salvador, BA, Brazil | pt_BR |
| Affilliation | São Rafael Hospital. Center for Biotechnology and Cell Therapy. Salvador, BA, Brazil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / São Rafael Hospital. Center for Biotechnology and Cell Therapy. Salvador, BA, Brazil | pt_BR |
| Subject | Chagas disease | pt_BR |
| Subject | Chagasic cardiomyopathy | pt_BR |
| Subject | Cells Transplantation | pt_BR |
| Subject | Trypanosoma cruzi | pt_BR |
| Subject | Animals | pt_BR |
| Subject | Mice | pt_BR |
| xmlui.metadata.dc.subject.ods | 03 Saúde e Bem-Estar | |
