Author | Lopes, Joao S | |
Author | Marques, Isabel | |
Author | Soares, Patricia | |
Author | Guimaraes, Hanna Nebenzahl | |
Author | Costa, Joao | |
Author | Miranda, Anabela | |
Author | Duarte, Raquel | |
Author | Alves, Adriana | |
Author | Macedo, Rita | |
Author | Duarte, Tonya Azevedo | |
Author | Bessa, Theolis Costa Barbosa | |
Author | Oliveira, Martha Maria de | |
Author | Nery, Joilda Silva | |
Author | Boechat, Neio | |
Author | Pereira, Susan Martins | |
Author | Barreto, Maurício Lima | |
Author | Leal, Jose Pereira | |
Author | Gomes, Maria Gabriela Miranda | |
Author | Goncalves, Carlos Penha | |
Access date | 2017-08-29T17:39:24Z | |
Available date | 2017-08-29T17:39:24Z | |
Document date | 2013 | |
Citation | LOPES, J. S. et al. SNP typing reveals similarity in Mycobacterium tuberculosis genetic diversity between Portugal and Northeast Brazil. Infection, Genetics and Evolution, v. 18, p. 238–246, 2013. | pt_BR |
ISSN | 1567-1348 | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/20823 | |
Description | a Instituto Gulbenkian de Ciencia, 2781-901 Oeiras, Portugal
b Instituto Nacional de Saude Dr. Ricardo Jorge, 4150-180 Porto, Portugal
c Centro Hospitalar de Vila Nova de Gaia/Espinho, 4434-502 Vila Nova de Gaia, Portugal
d Departamento de Epidemiologia Clinica, Medicina Preventiva e Saúde Pública, Faculdade de Medicina da Universidade do Porto, 4200-319 Porto, Portugal
e Direccao-Geral da Saude, 1049-005 Lisboa, Portugal
f Instituto de Doenças do Torax, Universidade Federal do Rio de Janeiro, 21.941-913 Rio de Janeiro, Brazil
g Instituto de Ciencias da Saude, Universidade Federal da Bahia, 40.150-510 Salvador, Brazil
h Centro de Pesquisas Goncalo Moniz, Fundacao Oswaldo Cruz, 40.296-710 Salvador, Brazil
i Centro de Pesquisa em Tuberculose, Universidade Federal do Rio de Janeiro, 21.941-913 Rio de Janeiro, Brazil | pt_BR |
Sponsorship | National Counsel of Technological and Scientific Development (CNPQ) [Project Number 410498/2006-8] and Coordination of Improvement of Higher Education Personnel (CAPES) [Project Number 23038.005107/2011-83]. | pt_BR |
Language | eng | pt_BR |
Publisher | Elsevier | pt_BR |
Rights | open access | pt_BR |
Subject in Portuguese | Mycobacterium tuberculosis | pt_BR |
Subject in Portuguese | Portugal | pt_BR |
Subject in Portuguese | Brasil | pt_BR |
Subject in Portuguese | Filogenia | pt_BR |
Title | SNP typing reveals similarity in Mycobacterium tuberculosis genetic diversity between Portugal and Northeast Brazil | pt_BR |
Type | Article | pt_BR |
DOI | 10.1016/j.meegid.2013.04.028 | |
Abstract | Human tuberculosis is an infectious disease caused by bacteria from the Mycobacterium tuberculosis complex (MTBC). Although spoligotyping and MIRU-VNTR are standard methodologies in MTBC genetic epidemiology, recent studies suggest that Single Nucleotide Polymorphisms (SNP) are advantageous in phylogenetics and strain group/lineages identification. In this work we use a set of 79 SNPs to characterize 1987 MTBC isolates from Portugal and 141 from Northeast Brazil. All Brazilian samples were further characterized using spolygotyping. Phylogenetic analysis against a reference set revealed that about 95% of the isolates in both populations are singly attributed to bacterial lineage 4. Within this lineage, the most frequent strain groups in both Portugal and Brazil are LAM, followed by Haarlem and X. Contrary to these groups, strain group T showed a very different prevalence between Portugal (10%) and Brazil (1.5%). Spoligotype identification shows about 10% of mis-matches compared to the use of SNPs and a little more than 1% of strains unidentifiability. The mis-matches are observed in the most represented groups of our sample set (i.e., LAM and Haarlem) in almost the same proportion. Besides being more accurate in identifying strain groups/lineages, SNP-typing can also provide phylogenetic relationships between strain groups/lineages and, thus, indicate cases showing phylogenetic incongruence. Overall, the use of SNP-typing revealed striking similarities between MTBC populations from Portugal and Brazil. | pt_BR |
Affilliation | Múltipla – ver em Notas | pt_BR |
Subject | Mycobacterium tuberculosis complex | pt_BR |
Subject | Portugal | pt_BR |
Subject | Brazil | pt_BR |
Subject | SNP-typing | pt_BR |
Subject | Spoligotyping | pt_BR |
Subject | Phylogeny | pt_BR |