| Author | Waniek, Peter J. | |
| Author | Castro, Helena C. | |
| Author | Sathler, Plínio C. | |
| Author | Miceli, Leonardo | |
| Author | Jansen, Ana M. | |
| Author | Araújo, Catarina A. C. | |
| Access date | 2017-08-29T17:56:17Z | |
| Available date | 2017-08-29T17:56:17Z | |
| Document date | 2009 | |
| Citation | WANIEK, Peter J. et al. Two novel defensin-encoding genes of the Chagas disease vector Triatoma brasiliensis (Reduviidae, Triatominae): Gene expression and peptide-structure modeling. Journal of Insect Physiology, v.55, p.840–848, 2009 | pt_BR |
| ISSN | 0022-1910 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/20825 | |
| Language | eng | pt_BR |
| Publisher | Elsevier | pt_BR |
| Rights | restricted access | |
| Subject in Portuguese | Trato digestivo | pt_BR |
| Subject in Portuguese | Triatoma | pt_BR |
| Subject in Portuguese | Imunidade aos insetos | pt_BR |
| Subject in Portuguese | Alimentação de sangue | pt_BR |
| Subject in Portuguese | Defensinas | pt_BR |
| Subject in Portuguese | Insetos | pt_BR |
| Title | Two novel defensin-encoding genes of the Chagas disease vector Triatoma brasiliensis (Reduviidae, Triatominae): gene expression and peptide-structure modeling | pt_BR |
| Type | Article | |
| DOI | 10.1016/j.jinsphys.2009.05.015 | |
| Abstract | Defensins are cysteine-rich peptides involved in the innate immunity of insects and many other organisms. In the present study, two novel defensin-encoding cDNAs and the respective genomic DNAs (def3 and def4) of Triatoma brasiliensis were identified and their tissue-specific and temporal expression was characterized. Both of the deduced mature peptides consisted of 43 amino acid residues and were highly similar to previously identified triatomine defensins (81.4-100%). Semi-quantitative RT-PCR data showed that def3 was constitutively expressed in the fat body and was induced in salivary glands and the small intestine at 5 and 3 days after feeding (daf), respectively. The def4 mRNA level was highly up-regulated in the stomach and fat-body tissues at 5 and 3 daf, respectively. The three-dimensional structures of these defensins were predicted using a homology modeling approach with Def-AAA, the defensin from Anopheles gambiae, as template (62-74% identity). A map of the electrostatic potential of these models revealed that, despite their similar folding patterns, mature Def2 and Def4 have a more cationic structure than is the case for Def1 and Def3. Such differences may orient the antimicrobial profile of these defensins against distinct targets in different organs of the insect. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia de Tripanosomatídeos. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Affilliation | Universidade Federal Fluminense. Instituto de Biologia. LaBioMol GCM. Niterói, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal Fluminense. Instituto de Biologia. LaBioMol GCM. Niterói, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal Fluminense. Instituto de Biologia. LaBioMol GCM. Niterói, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia de Tripanosomatídeos. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia de Tripanosomatídeos. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Subject | Defensin | pt_BR |
| Subject | Triatoma brasiliensis | pt_BR |
| Subject | Digestive tract | pt_BR |
| Subject | Insect immunity | pt_BR |
| Subject | Blood feeding | pt_BR |
| e-ISSN | 1879-1611 | |
| Embargo date | 2030-01-01 | |
| xmlui.metadata.dc.subject.ods | 02 Fome zero e agricultura sustentável | |
